Recombinant Anti-Podoplanin (MAP tag) (Clone PMab-1)

CAT#: TA386128

Recombinant Anti-Podoplanin (MAP tag) (Clone PMab-1)

Product Images

Flow-cytometry using the anti-Podoplanin (MAP tag) antibody PMab-1 (TA386128) NIH3T3 cells were stained with anti-Fluorescein IgG antibody (clone 4-4-20; isotype control, black line) or the rabbit IgG-chimeric version of PMab-1 (TA386128, blue line) at a dilution of 1:100 for 1h at RT. After washing, bound antibody was detected using a goat anti-rabbit IgG AlexaFluor® 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.

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  Western Blot using anti-Podoplanin (MAP tag) antibody PMab-1 (TA386128) Mouse thymus (A), mouse brain (B), NIH3T3 cells (C) and mouse kidney (D) lysates (35µg protein in RIPA buffer) were resolved on a 10% SDS PAGE gel and blots probed with the chimeric rabbit IgG version of 1B4 (TA386279) at 0.01 µg/ml, 0.1 µg/ml, 0.001 µg/ml and 0.003 µg/ml, respectively, before detection using an anti-rabbit secondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence.

Specifications

Product Data

• Clone Name: PMab-1
• Applications: Bl, ELISA, IHC, WB
• Target: MAP tag
• Host: Rabbit
• Clonality: Monoclonal
• Immunogen: This antibody was raised by immunising rats with 14-residue synthetic peptide mpp3851, which corresponds to amino acids 38-51 (GDGMVPPGIEDKIT) of the platelet-aggregation-stimulating (PLAG) domain of mouse podoplanin. Spleen cells were then harvested, and fused to P3U1 cells to generate stable hybridomas.
• Isotype: IgG, kappa
• Formulation: PBS with 0.02% Proclin 300.
• Concentration: lot specific
• Conjugation: Unconjugated
• Storage: Please store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C. Avoid freeze and thaw cycles.
• Stability: 3 years from dispatch.
• Gene Name: podoplanin
• Note: This chimeric rabbit antibody was made using the variable domain sequences of the original rat format, for improved compatibility with existing reagents, assays and techniques.