YFP Goat Polyclonal Antibody

CAT#: AB1166-100

Goat Polyclonal Anti-YFP Antibody

Product Images

Anti-YFP Ab at 1/2,000 dilution; 293HEK cells transduced with YFP Ad; lysates at 50 ug per lane; rabbit polyclonal to goat IgG (HRP) at 1/10,000 dilution;

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  Immunofluorescence – anti-YFP Ab in 293HEK cells transfected with YFP at 1/50 dilution; cells were fixed with 4% of PFA;

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  Immunogold labeling of epithelium cells, in vivo injected with YFP expressing vector;

Specifications

Product Data

• Applications: IF, WB
• Recommended Dilution: WB 1:500-1:2,000; IF 1:50-1:1,000; IHC (paraffin) 1:50-1:1,000; IHC (frozen) 1:50-1:1,000
• Target: YFP tag
• Host: Goat
• Clonality: Polyclonal
• Immunogen: Purified recombinant peptide produced in E. coli.
• Isotype: IgG
• Formulation: Polyclonal antibody supplied as a 100 µl (3 mg/ml) aliquot in PBS, 20% glycerol and 0.05% sodium azide.
• Concentration: 3mg/ml
• Purification: epitope affinity purified
• Conjugation: Unconjugated
• Background: Yellow Fluorescent Protein
• Note: In 293HEK cells transfected with cds plasmid detects a band of 27 kDa by Western blot. This antibody also recognizes GFP and does not recognize mCherry/red fluorescent protein.