Rabbit IgG (H&L) Antibody Alkaline Phosphatase Conjugated Pre-Adsorbed
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USD 200.00
Specifications
Product Data | |
Applications | ELISA, IHC, WB |
Recommended Dilution | WB: 1:500 - 1:2,000 IHC: 1:200 - 1:1,000 ELISA: 1:1,500 - 1:6,000 |
Host | Rat |
Clonality | Polyclonal |
Immunogen | Rabbit IgG whole molecule |
Specificity | This product was prepared from monospecific antiserum by immunoaffinity chromatography using Rabbit IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Alkaline Phosphatase (calf intestine), anti-Rat Serum, Rabbit IgG and Rabbit Serum. No reaction was observed against Goat, Human and Mouse Serum Proteins. Specificity was confirmed using ELISA at less than 1% cross reactivity against the sources listed above. |
Formulation | 0.05 M Tris Chloride, 0.15M Sodium Chloride, 0.001M Magnesium Chloride, 0.0001M Zinc Chloride, 50% (v/v) Glycerol; pH 8.0 |
Concentration | 1.0 mg/mL - lot specific |
Conjugation | Alkaline Phosphatase |
Storage | Store vial at 4° C before opening. DO NOT FREEZE. This product is stable at 4° C as an undiluted liquid. Dilute only prior to immediate use. Freezing alkaline phosphatase conjugates will result in a substantial loss of enzymatic activity. |
Note | Anti-Rabbit IgG antibody is suitable for use in ELISA, immunohistochemistry, and western blot. Specific conditions for reactivity should be optimized by the end user. |
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