Mouse IgG2b Antibody Texas Red™ Conjugated Pre-adsorbed
Frequently bought together (1)
beta Actin Mouse Monoclonal Antibody, Clone OTI1, Loading Control
USD 200.00
Specifications
Product Data | |
Applications | IF, IHC, WB |
Recommended Dilution | WB: 1:4,000 - 1:20,000 IHC: 1:1,000 - 1:5,000 IF: 1:1,000 - 1:5,000 FLISA: 1:10,000 - 1:50,000 |
Reactivities | Mouse |
Host | Goat |
Clonality | Polyclonal |
Immunogen | Mouse IgG2b heavy chain |
Specificity | Anti-MOUSE IgG2b (Gamma 2b chain) Antibody was prepared from monospecific antiserum by immunoaffinity chromatography using antigens coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum, Mouse Serum and Mouse IgG2b. No reaction was observed against Bovine, Human, and Rabbit Serum Proteins. Specificity was confirmed by ELISA at less than 1% of target signal. |
Formulation | 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 |
Reconstitution Method | Restore with deionized water (or equivalent) |
Concentration | 1.0 mg/mL - lot specific |
Conjugation | Texas Red |
Storage | Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. |
Note | Anti-Mouse IgG2b Texas Red™ Conjugated antibody has been tested by dot blot and is suitable for ELISA, Immunohistochemistry, western blotting, immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting as well as other anti IgG2a antibody based assays. Mouse IgG2b secondary antibody is available in a variety of formats. This product is suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms. |
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