Rat IgG (H+L chain), adsorbed Rabbit Polyclonal Antibody
CAT#: R1372F
Rat IgG (H+L chain), adsorbed rabbit polyclonal antibody, FITC
Conjugation: Unconjugated Biotin HRP
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Specifications
Product Data | |
Recommended Dilution | Suitable for Immunomicroscopy and Flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring lot-to-lot consistency. |
Reactivities | Rat |
Host | Rabbit |
Clonality | Polyclonal |
Immunogen | Rat IgG whole molecule. |
Specificity | This product was prepared from monospecific antiserum by immunoaffinity chromatography using Rat IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Fluorescein, anti-Rabbit Serum, Rat IgG and Rat Serum. No reaction was observed against Human Serum Proteins. |
Formulation | 0.01 M Sodium Phosphate, 0.14 M Sodium Chloride, pH 7.4 with 10 mg/ml Bovine Serum Albumin (BSA) IgG and Protease free as stabilizer and 0.01% (w/v) Thimerisol as preservative. Label: FITC State: Lyophilized purified Ig fraction. Label: Fluorescein isothiocyanate (Molecular Weight 390 daltons) Absorption emission: 495 nm / 528 nm Molar radio: 3.2 moles FITC per mole of Rabbit IgG. |
Reconstitution Method | Restore with 1.0 ml of deionized water (or equivalent). |
Concentration | lot specific |
Purification | Immunoaffinity chromatography. |
Conjugation | FITC |
Storage | Store vial at 2-8°C prior to restoration. For extended storage add glycerol to 50% and then aliquot contents and freeze at -20°C or below. Centrifuge product if not completely clear after standing at room temperature. This antibody is stable for one month at 2-8°C as an undiluted liquid. Dilute only prior to immediate use. Avoid repeated freezing and thawing. |
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