Mouse IgE (Fc specific) Goat Polyclonal Antibody

CAT#: AP21482TC-N

Mouse IgE (Fc specific) goat polyclonal antibody, TRITC

Specifications

Product Data

• Applications: ID, IF, IHC, IP
• Recommended Dilution: Can be used in Immunocytochemical and Immunohistochemical staining for the detection of IgE at the cellular and subcellular level by staining of appropriately treated cell and tissue substrates; to identify and measure IgE in mouse serum or other body fluids. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal.
  Recommended working dilutions: 1/20 - 1/60, depending on the method used.
• Reactivities: Mouse
• Host: Goat
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: Pools of purified homogenous IgE isolated from mouse serum. Freund’s complete adjuvant is used in the first step of the immunization procedure.
• Specificity: The reactivity of the antiserum is directed to the Fc subunit of the IgE molecule, which expresses strict (class) specificity. In immunoelectrophoresis and radial in immunodiffusion using various antiserum concentrations against serum of mice belonging to different allotypic groups, a single precipitin line has been obtained which shows a reaction of identity with the precipitin lines obtained with the purified IgE used as immunogens. It does not react with IgG including all subclasses, IgG/Fab fragments, IgM and IgA or any non-Ig protein in mouse serum, as tested by immunoelectrophoresis and double radial immunodiffusion. The antiserum also react with membrane-bound IgE in peripheral blood cells of different mouse strains as tested by immunofluorescence microscopy.
  Cross-reactivity: This immunoconjugate is not species-specific since inter-species cross-reactivity is a normal feature of antisera to immunoglobulins. However this conjugate has been passed over appropriate immuno-adsorbents to remove antibodies cross-reacting with mouse immunoglobulins. This renders it specific for use in test systems containing material of mouse origin (e.g. mouse tissue/mouse monoclonal antibody to a mouse tissue constituent/anti mouse Ig isotype-specific immunoconjugate.
• Formulation: PBS, pH 7.2 without preservatives or foreign proteins
  Label: TRITC
  State: Lyophilized purified Ig fraction
  Label: Tetramethylrhodamine Isothiocyanate
  Fluorescent marker: Tetramethylrhodamine isothiocyanate isomer R. It has an orange-red fluorescence. To avoid nonspecific background staining, specially synthesized and exceptionally pure crystalline isomer R has been used instead of the usual racemic mixture. Although its fluorescence efficiency is less than of FITC, conjugates have the advantage of significantly less photo bleaching. This facilitates their use in quantitative cell-counting procedures.
  Conjugation procedure: A proprietary technique for the binding of is used, followed by several purification steps to remove free reactants and protein aggregates. After each step activity and specificity are tested in a variety of techniques. The conjugate is lyophilized to assure stability and long shelf life
  Absorption emission: 554 nm / 573 nm
  Molar radio: Fluorochrome/IgG ~1.6
• Reconstitution Method: Restore by adding 1 ml sterile distilled water, spun down to remove insoluble particles, divided into small aliquots
• Concentration: N/A
• Purification: Hyperimmune antisera with strong precipitating activity are selected for fractionation and purification of the IgG (7S) fraction containing the bulk of the defined antibody specificity. It is free of other serum proteins as tested by immunoelectrophoresis.
• Conjugation: TRITC
• Storage: Prior to and following reconstitution store the antibody at 2-8°C for one month or at -20°C for longer.
  Avoid repeated freezing and thawing.
• Note: Adsorption: Immunoaffinity adsorbed using insolubilized antigens as required to eliminate antibodies cross-reacting with other components of the immunoglobulin system or reacting with other serum proteins. Special attention is given to the removal of antibodies to common Ig/Fab. The use of insolubilized adsorption antigens prevents the presence of excess adsorbent protein or immune complexes in the antiserum.