glucose 6 phosphatase, catalytic subunit (G6PC) Rabbit Monoclonal Antibody [Clone ID: OTIR4H3]

CAT#: TA592779

G6PC Rabbit monoclonal antibody,clone OTIR4H3

Product Images

IHC staining of FFPE human kidney tissue within the normal limits using anti-G6PC rabbit monoclonal antibody (TA592779) and Polink-2 HRP polymer detection kit (D22-110). Heat-induced epitope retrieval by EDTA solution buffer pH 8.0 (B04C-100) at 120°C for 3 min. The brown stain indicates positive stain, blue is the counter stain.

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  IHC staining of FFPE human liver tissue within the normal limits using anti-G6PC rabbit monoclonal antibody (TA592779) and Polink-2 HRP polymer detection kit (D22-110). Heat-induced epitope retrieval by EDTA solution buffer pH 8.0 (B04C-100) at 120°C for 3 min. The brown stain indicates positive stain, blue is the counter stain.

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  IHC staining of FFPE human duodenum tissue within the normal limits using anti-G6PC rabbit monoclonal antibody (TA592779) and Polink-2 HRP polymer detection kit (D22-110). Heat-induced epitope retrieval by EDTA solution buffer pH 8.0 (B04C-100) at 120°C for 3 min. The brown stain indicates positive stain, blue is the counter stain.

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  Western blot analysis of overexpressed lysates from HEK293T cells transfected with empty plasmid (PS100001, lane 1, 15ug) and human G6PC recombinant protein (lane 2, 100ng) using anti-G6PC antibody TA592779(1:3000).

Specifications

Product Data

• Clone Name: OTIR4H3
• Applications: IHC, WB
• Recommended Dilution: WB 1:2000, IHC 1:200-1:1000
• Reactivities: Human
• Host: Rabbit
• Isotype: IgG
• Clonality: Monoclonal
• Immunogen: Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human G6PC (NP_000142). The exact sequence is proprietary.
• Formulation: PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
• Concentration: 1 mg/ml
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Shipped at -20°C or with ice packs, Upon delivery store at -20°C. Dilute in PBS(pH7.3) if necessary. Stable for 12 months from date of receipt. Avoid repeated freeze-thaws.
• Predicted Protein Size: 40.5 kDa
• Gene Name: glucose-6-phosphatase catalytic subunit 1
• Database Link:
  NP_000142
  Entrez Gene 2538 Human
  UniProt ID P35575
• Background: Glucose-6-phosphatase (G6Pase) is a multi-subunit integral membrane protein of the endoplasmic reticulum that is composed of a catalytic subunit and transporters for G6P, inorganic phosphate, and glucose. This gene (G6PC) is one of the three glucose-6-phosphatase catalytic-subunit-encoding genes in human: G6PC, G6PC2 and G6PC3. Glucose-6-phosphatase catalyzes the hydrolysis of D-glucose 6-phosphate to D-glucose and orthophosphate and is a key enzyme in glucose homeostasis, functioning in gluconeogenesis and glycogenolysis. Mutations in this gene cause glycogen storage disease type I (GSD1). This disease, also known as von Gierke disease, is a metabolic disorder characterized by severe hypoglycemia associated with the accumulation of glycogen and fat in the liver and kidneys.[provided by RefSeq, Feb 2011]
• Synonyms: G6Pase; G6PC; G6PT; GSD1; GSD1a

Reference Data

• Protein Families: Druggable Genome, ES Cell Differentiation/IPS, Transmembrane
• Protein Pathways: Adipocytokine signaling pathway, Galactose metabolism, Glycolysis / Gluconeogenesis, Insulin signaling pathway, Metabolic pathways, Starch and sucrose metabolism