H3C14 Rabbit Polyclonal Antibody

CAT#: TA397503

Histone H3 K37me2 Antibody

Product Images

Immunofluorescence of Rabbit Anti-Histone H3 [Dimethyl Lys37] Antibody. Tissue: HeLa cells. Fixation: 0.5% PFA. Antigen retrieval: Not required. Primary antibody: Histone H3 [Dimethyl Lys37] antibody at a 1:100 dilution for 1 h at RT. Secondary antibody: Dylight 488 secondary antibody at 1:10,000 for 45 min at RT. Localization: Histone H3 [Dimethyl Lys37] is nuclear and chromosomal. Staining: Histone H3 [Dimethyl Lys37] is expressed in green, nuclei are counterstained with DAPI (blue).

Specifications

Product Data

• Applications: ChIP, IF, IHC, WB
• Recommended Dilution: WB: 1:500-1:2000
  IHC: 1:100
  IF: 1:100
  CHiP: 2-5µg/million cells
• Reactivities: C. elegans, Human, Mouse
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Histone H3 [Dimethyl Lys37] affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic dimethylated peptide surrounding Lysine 37 of human Histone H3.
• Specificity: Anti-Histone H3 [Dimethyl Lys37] was affinity purified from monospecific antiserum by immunoaffinity chromatography. This antibody reacts with human Histone H3. A BLAST analysis was used to suggest cross-reactivity with Human, mouse, and C. elegans. Predicted to react with many species including rat, chicken, Xenopus, Drosophila, and plant based on 100% sequence homology. Cross-reactivity with Histone H3 from other sources has not been determined.
• Formulation: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Concentration: 0.69 mg/mL - lot specific
• Conjugation: Unconjugated
• Storage: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
• Stability: Expiration date is one (1) year from date of receipt.
• Gene Name: histone cluster 2, H3c
• Database Link:
  Entrez Gene 333932 HumanEntrez Gene 126961 Human
  UniProt ID Q71DI3
• Background: Chromatin is the arrangement of DNA and proteins in which chromosomes are formed. Correspondingly, chromatin is formed from nucleosomes, which are comprised of a set of four histone proteins (H2A, H2B, H3, H4) wrapped with DNA. Chromatin is a very dynamic structure in which numerous post-translational modifications work together to activate or repress the availability of DNA to be copied, transcribed, or repaired. These marks decide which DNA will be open and commonly active (euchromatin) or tightly wound to prevent access and activation (heterochromatin). Common histone modifications include methylation of lysine and arginine, acetylation of lysine, phosphorylation of threonine and serine, and sumoylation, biotinylation, and ubiquitylation of lysine. Anti-Histone H3 are ideal for researchers interested in Chromatin Modifiers, Chromatin Research, Histones and Modified Histones, and Epigenetics research.
• Synonyms: rabbit anti-Histone H3 dimethyl Lys37 antibody, H3.3B, H3 histone, family 3A, H3.3AH3F3H3F3B, histone H3.3, MGC87782, MGC87783, H3K37me2
• Note: Anti-Histone H3 [Dimethyl Lys37] antibody is tested for Western Blot, Immunofluorescence, and Dot Blot. This antibody is useful for Chromatin Immunoprecipitation. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately ~15.4 kDa corresponding to Histone H3 protein by Western Blotting in HeLa histone prep lysate or the appropriate cell lysate or extract. Epi-Plus™ antibody production in collaboration with Novus Biologicals.

Reference Data

• Protein Pathways: Systemic lupus erythematosus