Alpha-amylase Rabbit Polyclonal Antibody

CAT#: TA396987S

Alpha Amylase Antibody Peroxidase Conjugated

Specifications

Product Data

• Applications: ELISA, IP, WB
• Recommended Dilution: WB: 1:500 - 1:5,000
  ELISA: 1:5,000 - 1:20,000
• Reactivities: Bacillus amyloliquefacies
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Anti-Alpha Amylase Antibody was produced by repeated immunizations with Bacillus amyloliquefaciens a-Amylase protein.
• Specificity: Alpha Amylase Antibody is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Rabbit Serum as well as purified and partially purified a-Amylase [Bacillus amyloliquefaciens]. Cross reactivity against a-Amylase from other tissues and species is unknown.
• Formulation: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Concentration: 1.0 mg/mL - lot specific
• Conjugation: HRP
• Storage: Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 µL). To minimize loss of volume dilute 1:10 by adding 225 µL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.
• Stability: Expiration date is one (1) year from date of receipt.
• Database Link:
  UniProt ID P00692
• Background: Anti-Alpha Amylase Antibody detects Alpha Amylase. α-Amylase is an enzyme that hydrolyses alpha-bonds of large alpha-linked polysaccharides, such as starch and glycogen, yielding glucose and maltose. Anti-Alpha Amylase Antibody is ideal for investigators involved in Cell Signaling.
• Synonyms: rabbit anti-Alpha Amylase Antibody Peroxidase Conjugation, HRP conjugated rabbit anti-Alpha Amylase Antibody, Alpha-amylase, 1,4-alpha-D-glucan glucanohydrolase
• Note: Alpha Amylase Peroxidase conjugate antibody has been tested by ELISA and western blot and is assayed against 1.0 ug of Alpha Amylase [Bacillus amyloliquefaciens] in a standard capture ELISA using ABTS (2,2’-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature. A working dilution of 1:2,000 to 1:10,000 of the reconstitution concentration is suggested for this product.