MAP2K2 Mouse Monoclonal Antibody [Clone ID: 12A6.G1.G11]

CAT#: TA396834S

MEK2 C-Term Antibody HRP

Product Images

Western Blot of Anti-MEK2 Antibody. Lane 1: MEK-1 recombinant protein. Lane 2: MEK-2 recombinant protein. Load: 50ng per lane. Primary Antibody: Anti-MEK2 supernatant clone neat over night at 4°C. Secondary Antibody: Anti-mouse HRP at 1:40,000 dilution.

Specifications

Product Data

• Clone Name: 12A6.G1.G11
• Applications: ELISA, WB
• Recommended Dilution: WB: 1µg/mL
  ELISA: 1:40,000
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG1, kappa
• Clonality: Monoclonal
• Immunogen: Anti-MEK2 Monoclonal Antibody was produced in mice by repeated immunizations with synthetic peptide corresponding to amino acid residues near the C-terminus conjugated to KLH.
• Specificity: This peroxidase conjugated protein A purified mouse monoclonal antibody reacts specifically with human MEK2. Anti-MEK2 is purified from tissue culture supernatant by protein A purification. Cross reactivity is expected to occur with human, mouse, and rat based on sequence identity of the peptide immunogen. This antibody does not react with the MEK1 isoform.
• Formulation: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Concentration: 1.0 mg/mL - lot specific
• Conjugation: HRP
• Storage: Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 µL). To minimize loss of volume dilute 1:10 by adding 225 µL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.
• Stability: Expiration date is one (1) year from date of receipt.
• Database Link:
  UniProt ID P36507
• Background: MEK2 antibodies detect the MEK2 isoform. Mitogen-activated protein kinase kinase 2, also known as MEK2 or MKK2, is an integral component of the MAP kinase cascade that regulates cell growth and differentiation. This pathway also plays a key role in synaptic plasticity in the brain. Activated MEK 2 acts as a dual specificity kinase phosphorylating both a threonine and a tyrosine residue on MAP kinase. MEK1 and MEK2 are about 80% identical to each other, and nearly identical within the kinase domain. This antibody does not react with MEK1. The MEK2 antibody is ideal for investigators involved in Neuroscience, Cell Signaling and Cancer Research.
• Synonyms: mouse anti-MEK2 Antibody HRP conjugation, peroxidase conjugated mouse anti-MEK2 Antibody, MAP2K2, MEK, MEK 2, MKK2, PRKMK2, CFC4, MEK-2 Antibody
• Note: Anti-MEK 2 Peroxidase Conjugated (MOUSE) Antibody is suitable for use in Western Blotting and ELISA. Specific conditions of reactivity should be optimized by the end user. Expect a band of approximately 44 kDa.