ycjM Goat Polyclonal Antibody
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Specifications
Product Data | |
Applications | ELISA, IP, WB |
Recommended Dilution | WB: 1:500 - 1:5,000 ELISA: 1:5,000 - 1:20,000 |
Reactivities | Escherichia coli |
Host | Goat |
Clonality | Polyclonal |
Immunogen | Sucrose Phosphorylase [E.coli] |
Specificity | Sucrose Phosphorylase is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Goat Serum as well as purified and partially purified Sucrose Phosphorylase [E.coli]. Cross reactivity against Sucrose Phosphorylase from other sources is unknown. |
Formulation | 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 |
Concentration | 1 mg/mL - lot specific |
Conjugation | HRP |
Storage | Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 µL). To minimize loss of volume dilute 1:10 by adding 225 µL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing. |
Stability | Expiration date is one (1) year from date of receipt. |
Database Link | |
Background | Sucrose Phosphorylase is an important enzyme in the metabolism of sucrose and regulation of other metabolic intermediates. Sucrose phosphorylase is in the class of hexosyltransferases. It has been shown in multiple experiments that the enzyme catalyzes this conversion by a double displacement mechanism. |
Synonyms | goat anti-Sucrose Phosphorylase Antibody, HRP Conjugated goat anti-Sucrose Phosphorylase Antibody, Putative sucrose phosphorylase, Sucrose glucosyltransferase |
Note | Anti-Sucrose Phosphorylase has been assayed against 1.0 µg of Sucrose Phosphorylase [E.coli] in a standard capture ELISA using ABTS (2,2’-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature. A working dilution of 1:1,000 to 1:5,000 of the reconstitution concentration is suggested for this product. |
Reference Data |
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