Tyrosine Hydroxylase (TH) Rabbit Polyclonal Antibody

CAT#: TA392937

TH (phospho-S8) polyclonal antibody

Product Images

Western blot (WB) analysis of p-TH (S8) pAb at 1:500 dilution Lane1:MCF-7 whole cell lysate(40ug) Lane2:MCF-7 treated with Forskolin(4µg/ml,30 minutes) whole cell lysate(40ug) Lane3:MCF-7 treated with Forskolin(4µg/ml,60 minutes) whole cell lysate(40ug)

Specifications

Product Data

• Applications: WB
• Recommended Dilution: WB: 1:500~1:1000
• Reactivities: Human, Mouse, Rat
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: Synthetic peptide derived from human TH around the phosphorylation site of S8.
• Specificity: TH (phospho-S8) polyclonal antibody detects endogenous levels of TH protein only when phosphorylated at Ser8.
• Formulation: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
• Concentration: 1mg/ml
• Conjugation: Unconjugated
• Storage: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
• Stability: 1 year
• Predicted Protein Size: ~ 58 kDa
• Gene Name: tyrosine hydroxylase
• Database Link:
  Entrez Gene 7054 Human
  UniProt ID P07101
• Background: The enzyme tyrosine hydroxylase (TH), also designated tyrosine 3-monooxygenase (TY3H), catalyzes the conversion of tyrosine to L-dopa, which is the rate limiting step in the biosynthesis of catecholamines such as dopamine, adrenalin and noradrenalin. TH is thought to play a role in the pathogenesis of Parkinson’s disease, which is associated with reduced dopamine levels. Two transcription factor binding sites in the proximal region of the TH gene, the TPA-responsive element (TRE) and the c-AMP responsive element (CRE), have been implicated in the complex regulation of the TH gene. TH is also known to be upregulated by the glia maturation factor (GMF), a Cdc 10/SWI6 motif-containing protein called V-1, and a variety of additional compounds
• Synonyms: TH; TYH; Tyrosine 3-hydroxylase; Tyrosine 3-monooxygenase
• Note: For research use only, not for use in diagnostic procedure.