SHP2 (PTPN11) Rabbit Polyclonal Antibody

CAT#: TA392895

SHP-2 (phospho-Y542) polyclonal antibody

Product Images

Western blot (WB) analysis of p-SHP-2 (Y542) polyclonal antibody at 1:500 dilution Lane1:A549 whole cell lysate Lane2:Raw264.7 whole cell lysate Lane3:H9C2 whole cell lysate

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  Immunohistochemistry (IHC) analyzes of p-SHP-2 (Y542) pAb in paraffin-embedded human breast cancer tissue.

Specifications

Product Data

• Applications: WB
• Recommended Dilution: WB: 1:500~1:1000 IHC: 1:50~1:200
• Reactivities: Human, Mouse, Rat
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: Synthetic phosphopeptide derived from human SHP-2 around the phosphorylation site of Tyrosine 542.
• Specificity: p-SHP-2 (Y542) polyclonal antibody detects endogenous levels of SHP-2 protein only when phosphorylated at Tyr542.
• Formulation: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
• Concentration: 1mg/ml
• Conjugation: Unconjugated
• Storage: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
• Stability: 1 year
• Predicted Protein Size: ~ 70 kDa
• Gene Name: protein tyrosine phosphatase, non-receptor type 11
• Database Link:
  Entrez Gene 5781 Human
  UniProt ID Q06124
• Background: Several groups have independently identified a non-transmembrane PTP, designated SH-PTP1 (also known as PTP1C, HCP and SHP), which is primarily expressed in hematopoietic cells and characterized by the presence of two SH2 domains N-terminal to the PTP domain. SH2 domains generally mediate the association of regulatory molecules with specific phosphotyrosine-containing sites on autophosphorylated receptors, thereby controlling the initial interaction of receptors with these substrates. A second and much more widely expressed PTP with SH2 domains, SH-PTP2 (also designated PTP1D and Syp), has been identified. Strong sequence similarity between SH-PTP2 and the Drosophila gene corkscrew (CSW) and their similar patterns of expression suggest that SH-PTP2 is the human corkscrew homolog.
• Synonyms: Protein-tyrosine phosphatase 1D; Protein-tyrosine phosphatase 2C; PTP-1D; PTP-2C; PTP2C; PTPN11; SH-PTP2; SH-PTP3; SHP-2; Shp2; SHPTP2; Tyrosine-protein phosphatase non-receptor type 11
• Note: For research use only, not for use in diagnostic procedure.