DARPP32 (PPP1R1B) Rabbit Polyclonal Antibody

CAT#: TA392887

DARPP-32 (phospho-T75) polyclonal antibody

Product Images

Western blot (WB) analysis of p-DARPP-32 (T75) pAb at 1:1000 dilution Lane1:AML-12 whole cell lysate(40ug) Lane2:C6 whole cell lysate(40ug) Lane3:HepG2 whole cell lysate(40ug) Lane4:SGC7901 whole cell lysate(40ug) Lane5:PC3 whole cell lysate(40ug)

• 

  Immunohistochemistry (IHC) analyzes of p-DARPP-32 (T75) pAb in paraffin-embedded human colorectal carcinoma tissue at 1:50.

Specifications

Product Data

• Applications: WB
• Recommended Dilution: WB: 1:500~1:1000 IHC: 1:50~1:200
• Reactivities: Human, Mouse, Rat
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: Synthetic phosphopeptide derived from human DARPP-32 around the phosphorylation site of Threonine 75.
• Specificity: p-DARPP-32 (T75) polyclonal antibody detects endogenous levels of DARPP-32 protein only when phosphorylated at Thr75
• Formulation: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
• Concentration: 1mg/ml
• Conjugation: Unconjugated
• Storage: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
• Stability: 1 year
• Predicted Protein Size: ~ 32 kDa
• Gene Name: protein phosphatase 1 regulatory inhibitor subunit 1B
• Database Link:
  Entrez Gene 84152 Human
  UniProt ID Q9UD71
• Background: Dopaminergic signaling pathways, which are essential for multiple brain functions, are abnormal in several neurological disorders, such as schizophrenia, Parkinson’s disease and drug abuse. DARPP-32 (for dopamine and adenosine 3’,5’-monophosphate-regulated phosphoprotein of 32 kDa) is abundant in neurons that receive dopaminergic input. Activation of PKA and the consequent phosphorylation of DARPP-32 on threonine occurs in response to dopamine acting upon D1-like receptors. Dopamine interaction with D2-like receptors results in the inhibition of PKA activation, the activation of protein phosphatase 2B and the consequent dephosphorylation of DARPP-32. Neurotransmitters other than dopamine may also be able to stimulate the phosphorylation or dephosphorylation of DARPP-32. Phosphorylated DARPP-32 is a potent inhibitor of PP-1.
• Synonyms: DARPP-32; DARPP32; Dopamine- and cAMP-regulated neuronal phosphoprotein; PPP1R1B; Protein phosphatase 1 regulatory subunit 1B
• Note: For research use only, not for use in diagnostic procedure.