Rabbit Polyclonal Antibody

CAT#: TA392643

AKT polyclonal antibody

Product Images

Western blot (WB) analysis of Akt polyclonal antibody at 1:500 dilution Lane1:L02 whole cell lysate(40ug) Lane2:A549 whole cell lysate(40ug) Lane3:HEK293T whole cell lysate(40ug) Lane4:C6 whole cell lysate(40ug) Lane5:BV2 whole cell lysate(40ug)

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  Immunohistochemistry (IHC) analyzes of AKT pAb in paraffin-embedded human kidney carcinoma tissue at 1:50,showing cytoplasm and nucleus staining.Negative control (the right)Using PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG-biotin followed by avidin-peroxidase.

Specifications

Product Data

• Applications: WB
• Recommended Dilution: WB: 1:500~1:1000 IHC: 1:50~1:200
• Reactivities: Human, Mouse, Rat
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: Recombinant full length Human AKT1.
• Specificity: AKT polyclonal antibody detects endogenous levels of total AKT protein.
• Formulation: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
• Concentration: 1mg/ml
• Conjugation: Unconjugated
• Storage: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
• Stability: 1 year
• Predicted Protein Size: ~ 60 kDa
• Database Link:
  UniProt ID P31749/P31751/Q9Y243
• Background: AKT, also known as protein kinase B (PKB), is a 57 kDa serine/threonine protein kinase. There are three mammalian isoforms of Akt: AKT1 (PKB alpha), AKT2 (PKB beta) and AKT3 (PKB gamma) with AKT2 and AKT3 being approximately 82% identical with the AKT1 isoform. Each isoform has a pleckstrin homology (PH) domain, a kinase domain and a carboxy terminal regulatory domain. AKT was originally cloned from the retrovirus AKT8, and is a key regulator of many signal transduction pathways. Its tight control over cell proliferation and cell viability are manifold; overexpression or inappropriate activation of AKT has been seen in many types of cancer.
• Synonyms: AKT1; AKT2; AKT3; PKB; PKB alpha; PKB beta; PKBG; PKB gamma; PKBα; PKBβ; PKBγ; Protein kinase Akt-2; Protein kinase Akt-3; Protein kinase B; Protein kinase B alpha; Protein kinase B beta; Protein kinase B gamma; Proto-oncogene c-Akt; RAC; RAC-alpha serine/threonine-protein kinase; RAC-beta serine/threonine-protein kinase; RAC-gamma serine/threonine-protein kinase; RAC-PK-alpha; RAC-PK-beta; RAC-PK-gamma; STK-2
• Note: For research use only, not for use in diagnostic procedure.