STAT3 Rabbit Polyclonal Antibody

CAT#: TA392588

Stat3 polyclonal antibody

Product Images

Western blot (WB) analysis of Stat3 pAb at 1:1000 dilution Lane1:H9C2 whole cell lysate(40ug) Lane2:AML-12 whole cell lysate(40ug) Lane3:MCF-7 whole cell lysate(40ug) Lane4:THP-1 whole cell lysate(40ug) Lane5:HepG2 whole cell lysate(40ug)

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  Immunofluorescence analysis of MCF-7 cells using Stat3 antibody at dilution of 1:50.

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  Immunoprecipitation of MCF-7 cell lysates using Stat3 pAb (Sepharose Bead Conjugate)#BD0048 (lane 4 and lane 5) and Nonspecific IgG Control (Sepharose Bead Conjugate)#BD0048 (lane 2 and lane 3) .Lane 1 is 30% input. The western blot was probed using Stat3 pAb #TA392588.

Specifications

Product Data

• Applications: WB
• Recommended Dilution: WB:1:500~1:1000 IP: 1:50~1:200 IF: 1:50~1:200
• Reactivities: Human, Mouse, Rat
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: Recombinant protein.
• Specificity: STAT3 polyclonal antibody detects endogenous levels of STAT3 protein.
• Formulation: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
• Concentration: 1mg/ml
• Conjugation: Unconjugated
• Storage: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
• Stability: 1 year
• Predicted Protein Size: ~ 80,89KDa
• Gene Name: signal transducer and activator of transcription 3
• Database Link:
  Entrez Gene 6774 Human
  UniProt ID P40763
• Background: Membrane receptor signaling by various ligands, including interferons and growth hormones such as EGF, induces activation of Jak kinases which then leads to tyrosine phosphorylation of the various Stat transcription factors. Stat1 and Stat2 are induced by IFN-α and form a heterodimer which is part of the ISGF3 transcription factor complex. Although early reports indicate Stat3 activation by EGF and IL-6, it has been shown that Stat3β appears to be activated by both while Stat3α is activated by EGF, but not by IL-6. Highest expresion of Stat4 is seen in testis and myeloid cells. IL-12 has been identified as an activator of Stat4. Stat5 has been shown to be activated by prolactin and by IL-3. Stat6 is involved in IL-4 activated signaling pathways.
• Synonyms: Acute-phase response factor; APRF; Signal transducer and activator of transcription 3; STAT-3
• Note: For research use only, not for use in diagnostic procedure.