MAPKAP Kinase 2 (MAPKAPK2) Rabbit Polyclonal Antibody

CAT#: TA392566

MAPKAPK-2 (Phospho-T334) polyclonal antibody

Product Images

Western blot (WB) analysis of MAPKAPK-2 (Phospho-T334) polyclonal antibody at 1:2000 dilution Lane1:HeLa whole cell lysate(40ug) Lane2:Hela treated with UV 5 min and PMA (200nM,10 min) whole cell lysate(40ug)

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  Immunofluorescence analysis of Hela cells using MAPKAPK-2 (Phospho-T334) antibody at dilution of 1:50.

Specifications

Product Data

• Applications: IF, WB
• Recommended Dilution: WB: 1:2000~1:5000 IF: 1:50~1:200
• Reactivities: Human
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: Synthetic phosphopeptide derived from human MAPKAPK-2 around the phosphorylation site of Threonine 334.
• Specificity: MAPKAPK-2 (Phospho-T334) polyclonal antibody detects endogenous levels of MAPKAPK-2 protein only when phosphorylated at Thr334.
• Formulation: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2.
• Concentration: 1mg/ml
• Conjugation: Unconjugated
• Storage: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
• Stability: 1 year
• Predicted Protein Size: ~ 48 kDa
• Gene Name: mitogen-activated protein kinase-activated protein kinase 2
• Database Link:
  Entrez Gene 9261 Human
  UniProt ID P49137
• Background: In response to cytokines, stress, and chemotactic factors, MAP kinase-activated protein kinase 2 (MAPKAPK-2) is rapidly phosphorylated and activated. It has been shown that MAPKAPK-2 is a direct target of p38 MAPK. Multiple residues of MAPKAPK-2 are phosphorylated in vivo in response to stress. However, only four residues (Thr25, Thr222, Ser272, and Thr334) are phosphorylated by p38 MAPK in an in vitro kinase assay. Phosphorylation at Thr222, Ser272, and Thr334 appears to be essential for the activity of MAPKAPK-2. Thr25 is phosphorylated by p42 MAPK in vitro, but is not required for the activation of MAPKAPK-2.
• Synonyms: MAPK-activated protein kinase 2; MAPKAP-K2; MAPKAPK-2; MAPKAPK2; MAPKAP kinase 2; MAP kinase-activated protein kinase 2; MK-2; MK2
• Note: For research use only, not for use in diagnostic procedure.