IKB alpha (NFKBIA) Rabbit Polyclonal Antibody

CAT#: TA392538

IKB-α (S32/S36) polyclonal antibody

Product Images

Western blot (WB) analysis of IKB-α (S32/S36) polyclonal antibody at 1:5000 dilution Lane1:CT-26 whole cell lysate(40ug) Lane2:PC12 whole cell lysate(40ug) Lane3:A549 whole cell lysate(40ug) Lane4:HepG2 whole cell lysate(40ug) Lane5:Hela whole cell lysate(40ug)

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  Immunohistochemistry of paraffin-embedded Rat Brain using IKB-α (S32/S36) antibody at dilution of 1:50.

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  Immunofluorescence analysis of A549 cells using IKB-α (S32/S36) antibody at dilution of 1:50.

Specifications

Product Data

• Applications: IF, IHC, WB
• Recommended Dilution: WB: 1:500~1:1000 IHC: 1:50~1:200 IF: 1:50~1:200
• Reactivities: Human, Mouse, Rat
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: Synthetic peptide, corresponding to Human IKB-α.
• Specificity: IKB-α (S32/S36) polyclonal antibody detects endogenous levels of IKB-α protein.
• Formulation: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
• Concentration: 1mg/ml
• Conjugation: Unconjugated
• Storage: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
• Stability: 1 year
• Predicted Protein Size: ~ 36 kDa
• Gene Name: NFKB inhibitor alpha
• Database Link:
  Entrez Gene 4792 Human
  UniProt ID P25963
• Background: The NF-κB/Rel transcription factors are present in the cytosol in an inactive state complexed with the inhibitory IκB proteins. Activation occurs via phosphorylation of IκBα at Ser32 and Ser36 followed by proteasome-mediated degradation that results in the release and nuclear translocation of active NF-κB. IκBα phosphorylation and resulting Rel-dependent transcription are activated by a highly diverse group of extracellular signals including inflammatory cytokines, growth factors, and chemokines. Kinases that phosphorylate IκB at these activating sites have been identified.
• Synonyms: I-kappa-B-alpha; IkappaBalpha; IkB-alpha; IKBA; MAD4; Major histocompatibility complex enhancer-binding protein MAD3; NF-kappa-B inhibitor alpha; NFKBI; NFKBIA
• Note: For research use only, not for use in diagnostic procedure.