p38 (MAPK14) Rabbit Polyclonal Antibody

CAT#: TA392535

p38 (V318) polyclonal antibody

Product Images

Western blot (WB) analysis of p38 (V318) polyclonal antibody at 1:1000 dillution Lane1:HEK293T whole cell lysate(36μg) Lane2:NIH-3T3 whole cell lysate(38.8μg) Lane3:HepG2 whole cell lysate(36.8μg)

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  Immunohistochemistry of paraffin-embedded Rat Brain using p38 (V318) antibody at dilution of 1:50.

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  Immunofluorescence analysis of U-87MG cells using p38 (V318) antibody at dilution of 1:50.

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  Immunoprecipitation of U-87MG cell lysates using p38 (V318) pAb (Sepharose Bead Conjugate)#BD0048 (lane 3 and lane 4) and Nonspecific IgG Control (Sepharose Bead Conjugate)#BD0048 (lane 1 and lane 2) .Lane 5 is 30% input. The western blot was probed using p38 (V318) pAb #TA392535.

Specifications

Product Data

• Applications: WB
• Recommended Dilution: WB: 1:1000~1:2000 IHC: 1:50~1:200 IF: 1:50~1:200 IP: 1:50~1:200
• Reactivities: Human, Mouse, Rat
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: Synthetic peptide, corresponding to Human p38.
• Specificity: p38 (V318) polyclonal antibody detects endogenous levels of p38 protein.
• Formulation: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
• Concentration: 1mg/ml
• Conjugation: Unconjugated
• Storage: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
• Stability: 1 year
• Predicted Protein Size: ~ 42 kDa
• Gene Name: mitogen-activated protein kinase 14
• Database Link:
  Entrez Gene 1432 Human
  UniProt ID Q16539
• Background: MAP (mitogen-activated protein) kinases play a significant role in many biological processes, including cell adhesion and spreading, cell differentiation and apoptosis. p38α, p38β and p38γ, also known as MAPK14, MAPK11 and MAPK12, respectively, each contain one protein kinase domain and belong to the MAP kinase family. Expressed in different areas throughout the body with common expression patterns in heart, p38 proteins use magnesium as a cofactor to catalyze the ATP-dependent phosphorylation of target proteins. Via their catalytic activity, p38α, p38β and p38γ are involved in a variety of events throughout the cell, including signal transduction pathways, cytokine production and cell proliferation and differentiation.
• Synonyms: CSAID-binding protein; CSBP; CSBP1; CSBP2; CSPB1; Cytokine suppressive anti-inflammatory drug-binding protein; MAPK14; MAPK 14; MAP kinase 14; MAP kinase MXI2; MAP kinase p38 alpha; MAX-interacting protein 2; Mitogen-activated protein kinase 14; Mitogen-activated protein kinase p38 alpha; MXI2; SAPK2A; SAPK2a; Stress-activated protein kinase 2a
• Note: For research use only, not for use in diagnostic procedure.