TEC Rabbit Polyclonal Antibody

CAT#: TA392486

TEC (Q75) polyclonal antibody

Product Images

Western blot (WB) analysis of TEC (Q75) polyclonal antibody at 1:2000 dilution Lane1:Hela whole cell lysate(40ug) Lane2:MCF-7 whole cell lysate(40ug) Lane3:CT-26 whole cell lysate(40ug) Lane4:PC12 whole cell lysate(40ug)

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  Immunofluorescence analysis of MCF-7 cells using TEC (Q75) antibody at dilution of 1:50.

Specifications

Product Data

• Applications: IF, WB
• Recommended Dilution: WB: 1:1000~1:2000 IF: 1:50~1:200
• Reactivities: Human, Rat, Mouse
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: Synthetic peptide, corresponding to Human TEC.
• Specificity: TEC (Q75) polyclonal antibody detects endogenous levels of TEC protein.
• Formulation: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2.
• Concentration: 1mg/ml
• Conjugation: Unconjugated
• Storage: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
• Stability: 1 year
• Predicted Protein Size: ~ 70 kDa
• Gene Name: tec protein tyrosine kinase
• Database Link:
  Entrez Gene 7006 Human
  UniProt ID P42680
• Background: Tec kinase belongs to a structurally related subfamily of protein tyrosine kinases (PTKs) that includes Btk, Itk (also known as Emt or Tsk), Bmx, and Txk (or Rlk). With the exception of Txk, the members of this subfamily possess a long amino-terminal region consisting of a pleckstrin homology (PH) domain and a Tec homology (TH) domain . Because PH domains bind phosphoinositides with high affinity, the Tec family kinases have been proposed to act downstream of phosphatidylinositol 3-kinase (PI3-kinase) in signaling pathways. Binding of the PH domain with phosphoinositides is probably required for targeting of Tec family kinases to the cell membrane. Tec kinase is activated in response to many upstream signaling events including antigen receptor, RTK, GPCR, and integrin stimulation. Activated Tec kinase directly phosphorylates substrates such as PLC-gamma 2 and BRDG1 docking protein and mediates downstream signaling.
• Synonyms: PSCTK4; TEC; Tyrosine-protein kinase Tec
• Note: For research use only, not for use in diagnostic procedure.