SRC Mouse Antibody [Clone ID: M259]

CAT#: TA389213

Anti-c-Src (N-terminal region) Antibody

Specifications

Product Data

• Clone Name: M259
• Applications: IP, WB
• Recommended Dilution: WB: 1:1000
• Reactivities: Human, Rat, Mouse
• Host: Mouse
• Isotype: IgG1
• Immunogen: Clone (M259) was generated from a recombinant protein containing amino acids in the N-terminal region of human c-Src. This sequence has high homology to similar regions in rat and mouse c-Src.
• Specificity: This antibody detects a 60 kDa* protein corresponding to c-Src on SDS-PAGE immunoblots of mouse SYF cells transformed with c-Src and human A431 cells.
• Formulation: PBS + 1 mg/ml BSA, 0.05% NaN3 and 50% glycerol
• Concentration: lot specific
• Purification: Protein A Purified
• Conjugation: Unconjugated
• Storage: Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.
• Stability: After date of receipt, stable for at least 1 year at -20°C.
• Predicted Protein Size: 60
• Database Link:
  UniProt ID P12931
• Background: c-Src was the first proto-oncogenic non-receptor tyrosine kinase characterized in human. The Src family is composed of nine members in vertebrates, including c-Src, Yes, Fgr, Yrk, Fyn, Lyn, Hck, Lck, and Blk. Src-family kinases transduce signals that are involved in the control of a variety of cellular processes, including proliferation, differentiation, motility, and adhesion. Src-family kinases contain an N-terminal cell membrane anchor followed by SH3 and SH2 domains. The activity of c-Src is regulated by tyrosine phosphorylation at multiple sites. Tyrosine 418 is autophosphorylated following c-Src activation. Tyrosine 215 in the SH2 domain of c-Src is phosphorylated following growth factor receptor activation. Both Tyr-215 and Tyr-418 phosphorylation increases tyrosine kinase activity, while phosphorylation of Tyr-530 downregulates c-Src kinase activity. Thus, tyrosine phosphorylation of c-Src is critical for regulating its kinase activity.
• Note: Protein G purified tissue culture supernatant.