Phospho-MAPK8 Mouse Antibody [Clone ID: M268]

CAT#: TA389158

Anti-JNK (Thr-183/Tyr-185), Phosphospecific Antibody

Specifications

Product Data

• Clone Name: M268
• Applications: ICC, WB
• Recommended Dilution: WB: 1:1000
  ICC: 1:100
• Reactivities: Human, Rat, Mouse
• Host: Mouse
• Isotype: IgG1
• Immunogen: Clone M268 was generated from a dual phosphorylated peptide corresponding to amino acid residues surrounding Thr-183 and Tyr-185 in human JNK1. This sequence has high homology to the conserved site in rat and mouse JNK1 and JNK2.
• Specificity: This antibody detects 46 and 54 kDa* proteins corresponding to the apparent molecular mass of JNK1 and JNK2 on SDS-PAGE immunoblots of human A431 and HeLa cells, as well as rat PC12 cells treated with calyculin A.
• Formulation: PBS + 1 mg/ml BSA, 0.05% NaN3 and 50% glycerol
• Concentration: lot specific
• Purification: Protein A Purified
• Conjugation: Unconjugated
• Storage: Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.
• Stability: After date of receipt, stable for at least 1 year at -20°C.
• Predicted Protein Size: 46/54
• Database Link:
  UniProt ID P45983
• Background: The stress-activated protein kinases (SAPK) or Jun-amino-terminal kinases (JNK) are potently activated by stressors such as UV and gamma radiation. Similar to other MAP Kinases, the core signaling unit is composed of a MAPKKK, usually MEKK1-4 or a mixed lineage kinase (MLK), which phosphorylate and activate MKK4-7, leading to dual phosphorylation and activation of JNK kinases. Rho-GTPases (Rac1 and cdc42) can stimulate MEKKs and MLKs, while MKKs can be activated by a GTPase-independent pathway that involves the germinal center kinase family. There are three JNK genes (JNK1, 2, 3) with further diversification resulting from alternative splicing. Active JNK dimers can translocate to the nucleus to regulate transcription through phosphorylation of c-Jun, ATF-2 and other transcription factors.
• Note: Protein G purified tissue culture supernatant.