ACTN4 Mouse Monoclonal Antibody [Clone ID: 93]

CAT#: TA388989

Anti-Alpha Actinin 4 Antibody

Specifications

Product Data

• Clone Name: 93
• Applications: IHC, WB
• Recommended Dilution: WB: 1:1000
  WB Brain: 1:1000
• Reactivities: Human, Mouse
• Host: Mouse
• Isotype: IgG
• Clonality: Monoclonal
• Immunogen: Fusion protein from the central rod domain of human α-actinin 4.
• Specificity: Specific for endogenous levels of the ~105 kDa α-actinin 4 protein.
• Formulation: 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg per ml BSA and 50% glycerol.
• Concentration: lot specific
• Purification: Protein G purified
• Conjugation: Unconjugated
• Storage: Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.
• Stability: After date of receipt, stable for at least 1 year at -20°C.
• Predicted Protein Size: 105
• Database Link:
  UniProt ID O43707
• Background: α-actinin-4 is a member of the actinin protein family comprised of an actin-binding domain in the N-terminus, 4 spectrin-like repeats in the central region, and 2 EF-hand motifs in the C-terminus (Honda et al, 1998). α-actinin-4 and CLP36 form a complex in normal kidney podocytes.CLP36 is dependent on α-actinin-4 for maintenance of its level in podocytes, whereas α-actinin-4 is independent of CLP36. α-actinin-4 is widely expressed in mammalian tissues and organs, while having a high occurrence of genetic mutations in kidney podocytes (Kos et al, 2003). FSGS, focal segmental glomerulosclerosis, is a rare genetic disease that attacks the kidney's filtering units (glomeruli) causing serious scarring which leads to permanent kidney damage and even failure. Three key mutations have been found in α-actinin-4 in people diagnosed with FSGS. R310Q and Q348R, located in the spectrin-like repeats region, and K255E located in the actin-binding region. The R310Q and Q348R mutation significantly inhibits the ability of α-actinin-4 to form the complex with CLP36. The K255E mutation was reversed where it increased the ability to bind CLP36 in the actin-binding region (Liu et al, 2011).
• Note: Protein G purified cultured supernatant.