B7-1 (CD80) Rabbit Monoclonal Antibody [Clone ID: IDEC-114 (Galiximab)]

CAT#: TA386090

Recombinant Anti-CD80 (Clone IDEC-114 (Galiximab))

Specifications

Product Data

• Clone Name: IDEC-114 (Galiximab)
• Applications: Bl, ELISA, FC, IHC, IP, WB
• Reactivities: Human
• Host: Rabbit
• Clonality: Monoclonal
• Immunogen: Galiximab was prepared by immunizing cynomolgus monkeys with recombinant CD80 antigen. The variable regions of the light and heavy chains were then cloned by being incorporated into a cassette vector (N5LG1) containing human constant region genes and subsequently transfected into the Dg44 CHO cell line (Hariharan K, 2013)
• Specificity: Galiximab is a primatized mAb which consists of human constant and primate (cynomolgus macaque) variable regions and binds specifically to CD80. CD80 is a surface glycoprotein and a member of the B7 family of costimulatory molecules. CD80 antigen regulates T cell activation (through interacting with CD28 or CD152) and is expressed transiently in antigen-presenting cells, T cells and normal B cells, and expressed consitutively on various subtypes of B-cell lymphomas.
  
  Galiximab acts to decrease in cell proliferation, inhibition of the constitutively active NF-κB pathway, increase in apoptosis and ADCC against various B-cell lymphoma cell lines. Galiximb affects CD28 and CD152 interactions with CD80. This antibody can also be used for FC, WB, ELISA, IP and IHC.
• Formulation: PBS with 0.02% Proclin 300.
• Concentration: lot specific
• Conjugation: Unconjugated
• Storage: Please store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C. Avoid freeze and thaw cycles.
• Stability: 3 years from dispatch.
• Gene Name: CD80 molecule
• Database Link:
  Entrez Gene 941 Human
  UniProt ID P33681
• Synonyms: B7; BB1; CD28LG; CD28LG1; LAB7
• Note: This chimeric rabbit antibody was made using the variable domain sequences of the original Human IgG1 format, for improved compatibility with existing reagents, assays and techniques.