CD44 Rat Monoclonal Antibody [Clone ID: Hermes-1]

CAT#: TA386029

Recombinant Anti-CD44 (Clone Hermes-1)

Specifications

Product Data

• Clone Name: Hermes-1
• Applications: Bl, ELISA, FC, IF, IHC, IP, Neutralize, WB
• Reactivities: Human, Mouse, Rat, Goat, Primate, Rabbit, Sheep, Bovine
• Host: Rat
• Isotype: IgG2a, kappa
• Clonality: Monoclonal
• Immunogen: This antibody was produced by immunizing a Fischer rat with human tonsil lymphocytes (10^7cells intraperitoneally in Freund's complete adjuvant). A boost was given 2 weeks later (intravascularly in saline).
• Specificity: This antibody is specific for CD44 (gp90 epitope cluster I), which is selectively expressed on normal or transformed lymphoid cells that are able to recognize and bind to high endothelial venules (specialized vessels that mediate lymphocyte exit from the blood into lymphoid organs). Although this antibody was raised against human CD44, it cross-reacts with murine, rat, rabbit, primate, sheep, bovine and goat CD44.
  
  This antibody binds CD44, so can be used to label CD44+ cells in flow cytometry analysis. As CD44 molecules are used to bind to high endothelial venules (HEV) at the site of lymphoctye entry to lymphoid organs, this antibody can be used to specifically stain HEV-binding cell lines (Jalkanen, 1986). In addition, this antibody blocks the adhesion of the haematopoietic progenitor cell line KG1 to hyaluronic acid (Bendall, 2003), but does not block lymphocyte binding to HEV (Picker, 1989). 7H9D6 partially blocks the binding of this antibody.
• Formulation: PBS with 0.02% Proclin 300.
• Concentration: lot specific
• Conjugation: Unconjugated
• Storage: Please store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C. Avoid freeze and thaw cycles.
• Stability: 3 years from dispatch.
• Gene Name: CD44 molecule (Indian blood group)
• Database Link:
  Entrez Gene 960 Human
  UniProt ID P16070
• Synonyms: CD44R; CDW44; CSPG8; ECMR-III; Epican; HCELL; HUTCH-I; IN; LHR; MC56; MDU2; MDU3; MGC10468; MIC4; MUTCH-I; PGP-1; PGP-I; Pgp1