Polysialic acid Rabbit Monoclonal Antibody [Clone ID: 735]

CAT#: TA385841

Recombinant Anti-Polysialic acid (Clone 735)

Product Images

Western Blot using anti-polysialic acid antibody TA385841 Rat brain lysate was resolved on a 10% SDS PAGE gel and blots probed with TA385841 at 2 µg/ml before being detected by a secondary antibody.

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  Immunohistochemical staining of guinea pig brain using anti-polysialic acid antibody TA385841 Formalin fixed guinea pig brain slices were were stained with a TA385841 at 3 µg/ml.

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  Immunohistochemical staining of rat spinal chord using anti-polysialic acid antibody TA385841 Formalin fixed rat spinal chord slices were were stained with a TA385841 at 3 µg/ml.

Specifications

Product Data

• Clone Name: 735
• Applications: FC, IHC, IP
• Reactivities: Human, Mouse, Rat
• Host: Rabbit
• Isotype: IgG, kappa
• Clonality: Monoclonal
• Immunogen: Homopolymers of α2,8-linked sialic acid of Escherichia coli K1.
• Specificity: Recognises Polysialic acid (PSA), a carbohydrate polymer attached to the neural cell adhesion molecule (NCAM), which shares molecular epitopes with homopolymers of α2,8-linked sialic acid of Escherichia coli K1.
  
  This antibody recognises an unusual postranslational modification on neural cell adhesion molecules important in synaptogenesis, oncogenesis (particularly small-cell lung cancer) and development of organs such as the kidney (studied in rats, Lackie et al. 1990). In addition to various studies on human cells, the antibody, has also been used the study the fine-composition of poly-sialic acid composition in mice (Galuska et al. 2006).
• Formulation: PBS with 0.02% Proclin 300.
• Concentration: lot specific
• Conjugation: Unconjugated
• Storage: Please store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C. Avoid freeze and thaw cycles.
• Stability: 3 years from dispatch.
• Note: This chimeric rabbit antibody was made using the variable domain sequences of the original murine IgG2a format, for improved compatibility with existing reagents, assays and techniques.