TRIM25 Rabbit Monoclonal Antibody [Clone ID: R06-1H2]

CAT#: TA385464

TRIM25 Rabbit monoclonal Antibody

Product Images

Western blot analysis of TRIM25/EFP in K562, rat Brain, C6, Hela lysates using TRIM25 antibody.

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  Immunocytochemistry analysis of TRIM25/EFP (green) in MCF-7 using TRIM25/EFP antibody,and DAPI(blue)

Specifications

Product Data

• Clone Name: R06-1H2
• Applications: IF, IP, WB
• Recommended Dilution: WB: 1/1000-1/5000
  ICC/IF: 1/50-1/200
  IP: 1/20
• Reactivities: Human, Rat
• Host: Rabbit
• Isotype: IgG
• Clonality: Monoclonal
• Immunogen: A synthetic peptide of human TRIM25
• Formulation: 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA
• Concentration: lot specific
• Purification: Affinity Purified
• Conjugation: Unconjugated
• Storage: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
• Stability: 1 year
• Predicted Protein Size: Calculated MW: 71 kDa; Observed MW: 71 kDa
• Gene Name: tripartite motif containing 25
• Database Link:
  Entrez Gene 7706 Human
  UniProt ID Q14258
• Background: Swiss-Prot Acc.Q14258.Functions as a ubiquitin E3 ligase and as an ISG15 E3 ligase. Involved in innate immune defense against viruses by mediating ubiquitination of DDX58. Mediates 'Lys-63'-linked polyubiquitination of the DDX58 N-terminal CARD-like region which is crucial for triggering the cytosolic signal transduction that leads to the production of interferons in response to viral infection. Promotes ISGylation of 14-3-3 sigma (SFN), an adapter protein implicated in the regulation of a large spectrum signaling pathway. Mediates estrogen action in various target organs. Mediates the ubiquitination and subsequent proteasomal degradation of ZFHX3 (PubMed:22452784).
• Synonyms: EFP; RNF147; Z147; ZNF147