RFWD3 Rabbit Polyclonal Antibody

CAT#: TA385164

RFWD3 Rabbit polyclonal Antibody

Product Images

Western blot analysis of RFWD3 in 823 lysates using RFWD3 antibody.

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  Western blot analysis of RFWD3 in MCF-7 lysates using RFWD3 antibody. The lane on the right is blocked with the synthesized peptide.

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  Western blot analysis of RFWD3 in HepG2 lysates using RFWD3 antibody.

Specifications

Product Data

• Applications: ELISA, IHC, WB
• Recommended Dilution: WB: 1/500-1/2000
  IHC: 1/100-1/300
  ELISA: 1/40000
• Reactivities: Human
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: The antiserum was produced against synthesized peptide derived from human RFWD3. AA range:374-423
• Formulation: Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide, pH 7.3.
• Concentration: lot specific
• Purification: Affinity Chromatography
• Conjugation: Unconjugated
• Storage: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
• Stability: 1 year
• Predicted Protein Size: Observed MW (kDa):85
• Database Link:
  UniProt ID Q6PCD5
• Background: Swiss-Prot Acc.Q6PCD5.E3 ubiquitin-protein ligase required for the repair of DNA interstrand cross-links (ICL) in response to DNA damage (PubMed:21504906, PubMed:21558276, PubMed:26474068, PubMed:28575657, PubMed:28575658). Plays a key role in RPA-mediated DNA damage signaling and repair (PubMed:21504906, PubMed:21558276, PubMed:26474068, PubMed:28575657, PubMed:28575658, PubMed:28691929). Acts by mediating ubiquitination of the RPA complex (RPA1, RPA2 and RPA3 subunits) and RAD51 at stalled replication forks, leading to remove them from DNA damage sites and promote homologous recombination (PubMed:26474068, PubMed:28575657, PubMed:28575658). Also mediates the ubiquitination of p53/TP53 in the late response to DNA damage, and acts as a positive regulator of p53/TP53 stability, thereby regulating the G1/S DNA damage checkpoint (PubMed:20173098). May act by catalyzing the formation of short polyubiquitin chains on p53/TP53 that are not targeted to the proteasome (PubMed:20173098). In response to ionizing radiation, interacts with MDM2 and enhances p53/TP53 ubiquitination, possibly by restricting MDM2 from extending polyubiquitin chains on ubiquitinated p53/TP53 (PubMed:20173098).