RAN Rabbit Monoclonal Antibody [Clone ID: R01-7J8]

CAT#: TA385128

Ran Rabbit monoclonal Antibody

Product Images

Western blot analysis of Ran in Raw264.7 lysates using Ran antibody.

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  Western blot analysis of Ran in Jurkat, Hela, COS7, Raji lysates using Ran antibody

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  Immunocytochemistry analysis of Ran (green) in U87-MG using Ran antibody,and DAPI(blue).

Specifications

Product Data

• Clone Name: R01-7J8
• Applications: IF, IP, WB
• Recommended Dilution: WB: 1/1000-1/5000
  ICC/IF: 1/50-1/200
  IP: 1/20-1/50
• Reactivities: Human, Mouse, Monkey
• Host: Rabbit
• Isotype: IgG
• Clonality: Monoclonal
• Immunogen: A synthetic peptide of human Ran
• Formulation: 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA
• Concentration: lot specific
• Purification: Affinity Purified
• Conjugation: Unconjugated
• Storage: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
• Stability: 1 year
• Predicted Protein Size: Calculated MW: 24 kDa; Observed MW: 24 kDa
• Gene Name: RAN, member RAS oncogene family
• Database Link:
  Entrez Gene 5901 Human
  UniProt ID P62826
• Background: Swiss-Prot Acc.P62826.GTPase involved in nucleocytoplasmic transport, participating both to the import and the export from the nucleus of proteins and RNAs (PubMed:10400640, PubMed:8276887, PubMed:8896452, PubMed:8636225, PubMed:8692944, PubMed:9351834, PubMed:9428644, PubMed:9822603, PubMed:26272610). Switches between a cytoplasmic GDP- and a nuclear GTP-bound state by nucleotide exchange and GTP hydrolysis (PubMed:7819259, PubMed:8896452, PubMed:8636225, PubMed:8692944, PubMed:9351834, PubMed:9428644, PubMed:9822603, PubMed:29040603, PubMed:11336674, PubMed:26272610). Nuclear import receptors such as importin beta bind their substrates only in the absence of GTP-bound RAN and release them upon direct interaction with GTP-bound RAN, while export receptors behave in the opposite way. Thereby, RAN controls cargo loading and release by transport receptors in the proper compartment and ensures the directionality of the transport (PubMed:8896452, PubMed:9351834, PubMed:9428644). Interaction with RANBP1 induces a conformation change in the complex formed by XPO1 and RAN that triggers the release of the nuclear export signal of cargo proteins (PubMed:20485264). RAN (GTP-bound form) triggers microtubule assembly at mitotic chromosomes and is required for normal mitotic spindle assembly and chromosome segregation (PubMed:10408446, PubMed:29040603). Required for normal progress through mitosis (PubMed:8421051, PubMed:12194828, PubMed:29040603). The complex with BIRC5/survivin plays a role in mitotic spindle formation by serving as a physical scaffold to help deliver the RAN effector molecule TPX2 to microtubules (PubMed:18591255). Acts as a negative regulator of the kinase activity of VRK1 and VRK2 (PubMed:18617507). Enhances AR-mediated transactivation. Transactivation decreases as the poly-Gln length within AR increases (PubMed:10400640).
• Synonyms: ARA24; Gsp1; OK/SW-cl.81; RanGTPase; TC4