RAD51 Rabbit Monoclonal Antibody [Clone ID: R06-5G2]

CAT#: TA385120

Rad51 Rabbit monoclonal Antibody

Product Images

Western blot detection of Rad51 in C6,3T3,Hela cell lysates using Rad51 Rabbit mAb(1:1000 diluted).Predicted band size:37kDa.Observed band size:37kDa.

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  Immunocytochemistry analysis of Rad51 (green) in Jurkat using Rad51 antibody,and DAPI(blue).

Specifications

Product Data

• Clone Name: R06-5G2
• Applications: IF, IP, WB
• Recommended Dilution: WB: 1/2000-1/10000
  ICC/IF: 1/200-1/100
  IP: 1/20
• Reactivities: Human, Mouse, Rat
• Host: Rabbit
• Isotype: IgG
• Clonality: Monoclonal
• Immunogen: A synthetic peptide of human Rad51
• Formulation: 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA
• Concentration: lot specific
• Purification: Affinity Purified
• Conjugation: Unconjugated
• Storage: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
• Stability: 1 year
• Predicted Protein Size: Calculated MW: 37 kDa; Observed MW: 37 kDa
• Gene Name: RAD51 recombinase
• Database Link:
  Entrez Gene 5888 Human
  UniProt ID Q06609
• Background: Swiss-Prot Acc.Q06609.Plays an important role in homologous strand exchange, a key step in DNA repair through homologous recombination (HR) (PubMed:28575658). Binds to single and double-stranded DNA and exhibits DNA-dependent ATPase activity. Catalyzes the recognition of homology and strand exchange between homologous DNA partners to form a joint molecule between a processed DNA break and the repair template. Binds to single-stranded DNA in an ATP-dependent manner to form nucleoprotein filaments which are essential for the homology search and strand exchange (PubMed:26681308). Part of a PALB2-scaffolded HR complex containing BRCA2 and RAD51C and which is thought to play a role in DNA repair by HR. Plays a role in regulating mitochondrial DNA copy number under conditions of oxidative stress in the presence of RAD51C and XRCC3. Also involved in interstrand cross-link repair (PubMed:26253028).
• Synonyms: BRCC5; HRAD51; HsRad51; HsT16930; RAD51A; RAD51D; RAD51L3; RECA