PAK2 Mouse Monoclonal Antibody [Clone ID: 7H3-E2-F5]

CAT#: TA384925

PAK2 (7H3) Mouse monoclonal Antibody

Product Images

Confocal immunofluorescence analysis of Hela cells using PAK2 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye.

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  Western blot detection of PAK2 in Jurkat,MCF7 and Hela cell lysates using PAK2 mouse mAb (1:5000 diluted).Predicted band size:61KDa.Observed band size:61KDa.

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  Immunohistochemical analysis of paraffin-embedded human lung cancer (left) and gastric cancer (right) using PAK2 mouse mAb with DAB staining.

Specifications

Product Data

• Clone Name: 7H3-E2-F5
• Applications: IF, IHC, WB
• Recommended Dilution: WB: 1/500-1/2000
  IHC: 1/200-1/1000
  ICC: 1/200-1/1000
• Reactivities: Human, Monkey
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Purified recombinant fragment of PAK2 expressed in E. Coli.
• Formulation: Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.03% Proclin 300, pH 7.3.
• Concentration: lot specific
• Purification: Ascitic Fluid
• Conjugation: Unconjugated
• Storage: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
• Stability: 1 year
• Predicted Protein Size: 61kDa
• Gene Name: p21 (RAC1) activated kinase 2
• Database Link:
  Entrez Gene 5062 Human
  UniProt ID Q13177
• Background: Swiss-Prot Acc.Q13177.PAK2, also known as P21 (CDKN1A)-activated kinase 2. The p21 activated kinases (PAK) are critical effectors that link Rho GTPases to cytoskeleton reorganization and nuclear signaling. The PAK proteins are a family of serine/threonine kinases that serve as targets for the small GTP binding proteins, CDC42 and RAC1, and have been implicated in a wide range of biological activities. PAK2 is activated by proteolytic cleavage during caspase-mediated apoptosis, and may play a role in regulating the apoptotic events in the dying cell. PAK2 has been shown to interact with SH3KBP1, CDC42 and Abl gene.
• Synonyms: Gamma-PAK; p58; PAK-2; PAK65; PAKgamma