Aspartate Aminotransferase (GOT1) Rabbit Monoclonal Antibody [Clone ID: R05-6C1]

CAT#: TA384313

Aspartate Aminotransferase Rabbit monoclonal Antibody

Product Images

Western blot analysis of Aspartate Aminotransferase in K562, rat Brain, 3T3, Hela lysates using Aspartate Aminotransferase antibody.

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  Immunohistochemistry analysis of paraffin-embedded Human colon cancer tissue using Aspartate Aminotransferase antibody. High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.

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  Immunocytochemistry analysis of Aspartate Aminotransferase (green) in U87-MG using Aspartate Aminotransferase antibody,and DAPI(blue).

Specifications

Product Data

• Clone Name: R05-6C1
• Applications: IF, IHC, WB
• Recommended Dilution: WB: 1/1000
  IHC: 1/20-1/100
  ICC/IF: 1/100-1/100
• Reactivities: Human, Mouse, Rat
• Host: Rabbit
• Isotype: IgG
• Clonality: Monoclonal
• Immunogen: A synthetic peptide of human Aspartate Aminotransferase
• Formulation: 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA
• Concentration: lot specific
• Purification: Affinity Purified
• Conjugation: Unconjugated
• Storage: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
• Stability: 1 year
• Predicted Protein Size: Calculated MW: 46 kDa; Observed MW: 46 kDa
• Gene Name: glutamic-oxaloacetic transaminase 1
• Database Link:
  Entrez Gene 2805 Human
  UniProt ID P17174
• Background: Swiss-Prot Acc.P17174.Biosynthesis of L-glutamate from L-aspartate or L-cysteine. Important regulator of levels of glutamate, the major excitatory neurotransmitter of the vertebrate central nervous system. Acts as a scavenger of glutamate in brain neuroprotection. The aspartate aminotransferase activity is involved in hepatic glucose synthesis during development and in adipocyte glyceroneogenesis. Using L-cysteine as substrate, regulates levels of mercaptopyruvate, an important source of hydrogen sulfide. Mercaptopyruvate is converted into H2S via the action of 3-mercaptopyruvate sulfurtransferase (3MST). Hydrogen sulfide is an important synaptic modulator and neuroprotectant in the brain.
• Synonyms: GIG18