PERK (EIF2AK3) Rabbit Monoclonal Antibody [Clone ID: R09-1F9]

CAT#: TA384148

PERK Rabbit monoclonal Antibody

Product Images

Western blot analysis of PERK in K562, C6, 3T3, Hela lysates using PERK antibody.

Specifications

Product Data

• Clone Name: R09-1F9
• Applications: WB
• Recommended Dilution: WB: 1/1000
• Reactivities: Human, Mouse, Rat
• Host: Rabbit
• Isotype: IgG
• Clonality: Monoclonal
• Immunogen: Recombinant protein of human PERK
• Formulation: 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA
• Concentration: lot specific
• Purification: Affinity Purified
• Conjugation: Unconjugated
• Storage: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
• Stability: 1 year
• Predicted Protein Size: Calculated MW: 125 kDa; Observed MW: 140 kDa
• Gene Name: eukaryotic translation initiation factor 2 alpha kinase 3
• Database Link:
  Entrez Gene 9451 Human
  UniProt ID Q9NZJ5
• Background: Swiss-Prot Acc.Q9NZJ5.Metabolic-stress sensing protein kinase that phosphorylates the alpha subunit of eukaryotic translation initiation factor 2 (eIF-2-alpha/EIF2S1) on 'Ser-52' during the unfolded protein response (UPR) and in response to low amino acid availability. Converts phosphorylated eIF-2-alpha/EIF2S1 either in a global protein synthesis inhibitor, leading to a reduced overall utilization of amino acids, or to a translation initiation activator of specific mRNAs, such as the transcriptional activator ATF4, and hence allowing ATF4-mediated reprogramming of amino acid biosynthetic gene expression to alleviate nutrient depletion. Serves as a critical effector of unfolded protein response (UPR)-induced G1 growth arrest due to the loss of cyclin-D1 (CCND1). Involved in control of mitochondrial morphology and function.
• Synonyms: DKFZp781H1925; HRI; HsPEK; PEK; PERK; WRS