Amyloid Precursor Protein (APP) Rabbit Monoclonal Antibody [Clone ID: R09-3F7]

CAT#: TA383742

Amyloid Precursor Protein Rabbit monoclonal Antibody

Product Images

Western blot analysis of Amyloid Precursor Protein in mouse brain lysates using Amyloid Precursor Protein antibody.

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  Western blot analysis of Amyloid Precursor Protein in rat Brain lysates using Amyloid Precursor Protein antibody.

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  Immunohistochemistry analysis of paraffin-embedded Human breast cancer using Amyloid Precursor Protein antibody. High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.

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  Immunocytochemistry analysis of Amyloid Precursor Protein(green) in Hela using Amyloid Precursor Protein antibody,and DAPI(blue).

Specifications

Product Data

• Clone Name: R09-3F7
• Applications: IF, IHC, WB
• Recommended Dilution: WB: 1/1000-1/5000
  IHC: 1/20
  ICC/IF: 1/20
• Reactivities: Human, Mouse, Rat
• Host: Rabbit
• Isotype: IgG
• Clonality: Monoclonal
• Immunogen: A synthetic peptide of human Amyloid Precursor Protein
• Formulation: 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA
• Concentration: lot specific
• Purification: Affinity Purified
• Conjugation: Unconjugated
• Storage: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
• Stability: 1 year
• Predicted Protein Size: Calculated MW: 87 kDa; Observed MW: 100 kDa
• Gene Name: amyloid beta precursor protein
• Database Link:
  Entrez Gene 351 Human
  UniProt ID P05067
• Background: Swiss-Prot Acc.P05067.Functions as a cell surface receptor and performs physiological functions on the surface of neurons relevant to neurite growth, neuronal adhesion and axonogenesis. Involved in cell mobility and transcription regulation through protein-protein interactions. Can promote transcription activation through binding to APBB1-KAT5 and inhibits Notch signaling through interaction with Numb. Couples to apoptosis-inducing pathways such as those mediated by G(O) and JIP. Inhibits G(o) alpha ATPase activity . Acts as a kinesin I membrane receptor, mediating the axonal transport of beta-secretase and presenilin 1. Involved in copper homeostasis/oxidative stress through copper ion reduction. In vitro, copper-metallated APP induces neuronal death directly or is potentiated through Cu2+-mediated low-density lipoprotein oxidation. Can regulate neurite outgrowth through binding to components of the extracellular matrix such as heparin and collagen I and IV. The splice isoforms that contain the BPTI domain possess protease inhibitor activity. Induces a AGER-dependent pathway that involves activation of p38 MAPK, resulting in internalization of amyloid-beta peptide and leading to mitochondrial dysfunction in cultured cortical neurons. Provides Cu2+ ions for GPC1 which are required for release of nitric oxide (NO) and subsequent degradation of the heparan sulfate chains on GPC1.
• Synonyms: A4; AAA; ABPP; AD1; APPI; CTFgamma; CVAP; OTTHUMP00000096096; PN-II; PN2; PreA4