S Protein Rabbit Polyclonal Antibody

CAT#: TA381943

SARS-CoV-2 Spike S2 ECD Rabbit polyclonal Antibody

Product Images

Immobilized Recombinant SARS-COV2 S-ECD-PFS Protein(RP01283LQ) at 1μg/mL (100μL/well) can bind SARS-CoV-2 Spike S2 ECD Rabbit pAb (TA381943) with a linear range of 3.12-50ng/mL.

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  Western blot analysis of extracts of normal 293T cells 293T transfected with Spike S2 ECD Protein, using SARS-CoV-2 Spike S2 ECD antibody (TA381943) at 1:5000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Enhanced Kit .|Exposure time: 1s.

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  Immunofluorescence analysis of 293T cells transfected with SARS-CoV-2 Spike S2 ECD fusion protein (top left) and untreated 293T cells (top right) use SARS-CoV-2 Spike S2 ECD Rabbit pAb (TA381943) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

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  Immunoprecipitation analysis of 300ug extracts of 293T cells using 3ug SARS-CoV-2 Spike S2 ECD antibody (TA381943). Western blot was performed from the immunoprecipitate using SARS-CoV-2 Spike S2 ECD antibody (TA381943) at a dilition of 1:5000.

Specifications

Product Data

• Applications: Dot, ICC/IF, IP, WB
• Recommended Dilution: Elisa,1:500 - 1:2000
  WB,1:500 - 1:2000
  FC,1:50 - 1:200
  IF,1:50 - 1:200
• Reactivities: SARS-CoV-2, Human
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: Recombinant fusion protein of SARS-CoV-2 Spike S2 ECD.
• Formulation: Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
• Concentration: lot specific
• Purification: Affinity purification
• Conjugation: Unconjugated
• Storage: Store at -20℃. Avoid freeze / thaw cycles.
• Stability: Shelf life: one year from despatch.
• Gene Name: S Protein
• Database Link:
  Entrez Gene 43740568 SARS-CoV-2
  UniProt ID P0DTC2
• Background: The spike protein (S) of coronavirus (CoV) attaches the virus to its cellular receptor, angiotensin-converting enzyme 2 (ACE2). A defined receptor-binding domain (RBD) on S mediates thisinteraction.The S protein plays key parts in the induction of neutralizing-antibody and T-cellresponses, as well as protective immunity.