Caspase-7 (CASP7) (C-term) Rabbit Polyclonal Antibody

CAT#: SP2148P

Caspase-7 (CASP7) (C-term) rabbit polyclonal antibody, Purified

Product Images

Western blot analysis of human skeletal muscle probed with Rabbit anti Human caspase-7 at 0.5 (A), 1 (B) and 2 (C) ug/ml

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  Immunohistochemical staining of human skeletal muscle with Rabbit anti Human caspase-7

Specifications

Product Data

• Applications: IHC, WB
• Recommended Dilution: Western blot: 1.0-2.0 µg/ml.
  Immunohistochemistry on Paraffin Sections: 2.0 µg/ml.
  This product does not require protein digestion pre-treatment of paraffin sections eg. Trypsin or pronase prior to staing.
  This product does not require antigen retrieval using heat treatment prior to staining of paraffin sections.
  Recommended Positive Control: Skeletal muscle tissue.
• Reactivities: Human, Mouse, Rat
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: A 16 amino acid peptide from Human Caspase-7 carboxy-terminus
• Specificity: This antibody recognises an epitope within the C-terminal region (CT) of Caspase-7, a 35kDa cysteine protease, otherwise known as ICE-like Apoptotic Protease 3 (ICE-LAP3) Caspase-7.
• Formulation: PBS
  State: Purified
  State: Liquid purified IgG fraction
  Preservative: 0.02% Sodium Azide
• Concentration: lot specific
• Purification: Affinity Chromatography
• Conjugation: Unconjugated
• Storage: Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Gene Name: caspase 7
• Database Link:
  Entrez Gene 840 Human
  UniProt ID P55210
• Background: ICE-LAP3 is a member of the ICE/Ced-3 subfamily, is an executioner caspase which undergoes proteolytic cleavage of its precursor to form active p12 and p20 subunits. Evidence that activation of Caspase-7 occurs during cell death induced by the cytokine death receptors Fas/APO-1 and the receptor of tumour necrosis factor (TNFR-1), coupled with the fact that granzyme-B activated Caspase-7 cleaves the nuclear enzyme poly (ADPribose) polymerase (PARP), suggests an important role for Caspase-7 in both cytokine-mediated and Granzyme-B mediated apoptosis pathways.
• Synonyms: CASP-7, CASP7, MCH3, CMH-1