Caspase 1 (CASP1) (C-term) Rabbit Polyclonal Antibody

CAT#: SP2146P

Caspase 1 (CASP1) (C-term) rabbit polyclonal antibody, Purified

Product Images

Western blot analysis of human heart lysate probed with Rabbit anti Human caspase-1 at 0.5 (A), 1 (B) and 2 (C) ug/ml

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  Immunohistochemical staining of human heart with Rabbit anti Human caspase-1

Specifications

Product Data

• Applications: IHC, WB
• Recommended Dilution: Western blot: 0.5 µg - 1.0 µg/ml.
  Immunohistochemistry on paraffin sections: 0.5 µg - 1.0 µg/ml; This product does not require protein digestion pre-treatment of paraffin embedded sections e.g. trypsin or pronase prior to staining.
  Recommended Positive Control: Human heart tissue.
• Reactivities: Human
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: A 16 amino acid peptide located near human Caspase-1 carboxy terminus.
• Specificity: This antibody recognises an epitope within the C-terminal region (CT) of Caspase-1, otherwise known as IL-1Beta converting enzyme. This antibody detects a cleaved subunit band of approximately 21 kDa in heart cell lysates (predicted precursor MWT 45.2kDa).
• Formulation: PBS containing 0.02% Sodium Azide
  State: Purified
  State: Liquid purified IgG
• Concentration: lot specific
• Purification: Affinity chromatography
• Conjugation: Unconjugated
• Storage: Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Gene Name: caspase 1
• Database Link:
  Entrez Gene 834 Human
  UniProt ID P29466
• Background: Caspase-1 is an intracellular cysteine protease, identified as a mammalian homologue to
  C. elegans cell death gene (ced-3). Caspase-1 has been classified as an inflammatory, rather than apoptotic caspase, due to its essential role in the cleavage of the inactive precursors of the cytokines IL-1beta and IL-18, into their mature activated and secretable forms. This regulation of pro-inflammatory cytokines by Caspase-1 has made inhibitors of Caspase-1 a possible target for use as therapeutic drugs for the treatment of inflammatory diseases.
• Synonyms: CASP-1, CASP1, IL1BC, IL1BCE, ICE, p45