Endo G (ENDOG) Rabbit Polyclonal Antibody

CAT#: SP1412P

Endo G (ENDOG) rabbit polyclonal antibody, Purified

Product Images

Western blot analysis of whole cell lysates from mouse 3T3 (M) and Human HepG2 (H) cells probed with Rabbit anti endonuclease G

• 

  EndoG - staining of human pancreas with anti-EndoG at 15 ug/ml. Formalin fixed paraffin processed tissue.

Specifications

Product Data

• Applications: IHC, WB
• Recommended Dilution: Western blot: 1 µg/ml - 2 µg/ml; this antibody detects a band of approximately 35kDa in HepG2 and 3T3 cell lysates.
  Immunohistochemistry on paraffin sections: 15 µg/ml; Sodium citrate buffer pH6.0 is recommended for this purpose.
  Recommended Positive Control: Human pancreas.
• Reactivities: Human, Mouse, Rat
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Synthetic peptide corresponding to amino acids 55 - 70 of endonuclease G.
• Specificity: This antibody recognises endonuclease G (endoG).
• Formulation: PBS containing 0.09% Sodium Azide
  State: Purified
  State: Liquid purified IgG
• Concentration: lot specific
• Purification: Affinity chromatography
• Conjugation: Unconjugated
• Storage: Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Gene Name: endonuclease G
• Database Link:
  Entrez Gene 2021 Human
  UniProt ID Q14249
• Background: Endonuclease G is a mitochondrion-specific nuclease that translocates to the nucleus and cleaves chromatin DNA during apoptosis. endoG is reported to act independently of caspases and may represent a caspase independent apoptotic pathway initiated from the mitochondria.
• Synonyms: ENDOG