Integrin alpha 6 (ITGA6) Mouse Monoclonal Antibody [Clone ID: Mab-5A]

CAT#: SM804R

Integrin alpha 6 (ITGA6) mouse monoclonal antibody, clone Mab-5A, PE, Purified

Specifications

Product Data

• Clone Name: Mab-5A
• Applications: FC
• Recommended Dilution: Flow Cytometry: Use 10 µl of neat antibody to label 10⁶ cells in 100 µl.
• Reactivities: Rat
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Rat oesophageal carcinoma cell line B2T.
  Spleen cells from immunised BALB/c mice were fused with cells of the mouse SP2/0 myeloma cell line.
• Specificity: This CD49f antibody, clone Mab-5A recognizes Rat CD49f, also known as Integrin alpha-6, VLA-6 or CD49 antigen-like family member F. CD49f is a 1130 amino acid ~130 kDa single pass type 1 transmembrane glycoprotein which functions as a receptor for laminin when in association with either beta-1 or beta-4 integrins on platelets and epithelial cells respectively.
  Clone Mab-5A does not react with Rat fibroblasts.
• Formulation: PBS
  Label: PE
  State: Purified
  State: Lyophilized purified IgG fraction
  Stabilizer: 1% BSA, 5% Sucrose
  Preservative: 0.09% Sodium Azide
• Reconstitution Method: Restore with 1.0ml distilled water
• Purification: Affinity Chromatography on Protein G
• Conjugation: PE
• Storage: Store lyophilized at 2-8°C for 6 months or at -20°C long term.
  After reconstitution store the antibody undiluted at 2-8°C for one month
  or (in aliquots) at -20°C long term.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Gene Name: integrin subunit alpha 6
• Database Link:
  UniProt ID P23229
• Background: CD49f is a 120 kD integrin family member, also known as VLA-6 alpha chain and alpha6 integrin subunit. CD49f associates with either integrin beta1 (CD29) or integrin beta4 (CD104) to form receptors (VLA-6 or alpha6 beta4 complex) for laminin and kalinin. CD49f is expressed on platelets, monocytes, T cells, placental trophoblasts, epithelial and endothelial cells. CD49f is involved in adhesion and can act as a co-stimulatory molecule for T cell activation and proliferation.
• Synonyms: Integrin alpha-6, VLA-6, VLA6