GST-Tag Mouse Monoclonal Antibody [Clone ID: 1E5]

CAT#: SM6024

GST-Tag mouse monoclonal antibody, clone 1E5, Purified

Product Images

Western blot analysis: Recombinant GST (28 kDa) and GST-fusion protein (61 kDa) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-GST antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugat

Specifications

Product Data

• Clone Name: 1E5
• Applications: ELISA, IF, WB
• Recommended Dilution: ELISA.
  Western blot (1/500-1/2,000), recommended starting dilution is 1/1,000.
  Immunofluorescence/Immnunocytochemistry.
• Reactivities: Schistosoma japonicum
• Host: Mouse
• Isotype: IgG2b
• Clonality: Monoclonal
• Immunogen: Recombinant Glutathione S transferase (GST) purified from E. coli
• Specificity: This antibody reacting specifically with GST will be useful in various immunotechniques to identify the expression of a GST fusion protein in bacteria, bacterial lysates or cells and tissues transfected with a GST fusion protein expressing vectors.
• Formulation: PBS, pH 7.4 containing 0.02% Sodium Azide and 10% Glycerol
  State: Purified
  State: Liquid purified Ig fraction
• Concentration: lot specific
• Purification: Affinity Chromatography on Protein G
• Conjugation: Unconjugated
• Storage: Store undiluted at 2-8°C for up to two weeks or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Database Link:
  UniProt ID P08515
• Background: Glutathione S transferases (GSTs) are a family of enzymes that play an important role in detoxification by catalyzing the conjugation of many hydrophobic and electrophilic compounds with reduced glutathione. GST proteins can be purified by immobilized glutathione affinity chromatography. Many recombinant proteins have been engineered with GST tags to facilitate the detection, isolation and purification of these proteins.
• Synonyms: Glutathione S-transferase Tag, GST26-Tag