Adiponectin (ADIPOQ) Mouse Monoclonal Antibody [Clone ID: 5H7]

CAT#: SM6014

Adiponectin (ADIPOQ) mouse monoclonal antibody, clone 5H7, Purified

Product Images

Western blot analysis: The extract of mouse liver was resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human adiponectin antibody (1:2000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Arrow indicates the oligomer of mouse adiponectinprotein in mouse liver.

Specifications

Product Data

• Clone Name: 5H7
• Applications: ELISA, WB
• Recommended Dilution: ELISA.
  Western blot (1:500 - 1:2,000), recommended starting dilution is 1:1000.
• Reactivities: Human, Mouse
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Recombinant human adiponectin (15-244 aa) purified from E. coli
• Specificity: This antibody clone 5H7 is specific for the collagen-like domain of adiponectin.
• Formulation: PBS, pH 7.4 containing 0.02% Sodium Azide and 10% Glycerol
  State: Purified
  State: Liquid purified Ig fraction
• Concentration: lot specific
• Purification: Protein-G affinity chromatography
• Conjugation: Unconjugated
• Storage: Store undiluted at 2-8°C for up to two weeks or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Gene Name: adiponectin, C1Q and collagen domain containing
• Database Link:
  Entrez Gene 9370 Human
  UniProt ID Q15848
• Background: Human Adiponectin, also referred to as AdipoQ, Acrp30, apm-1 or GBP28, is a secreted protein expressed exclusively in differentiated adipocyte (an adipokine family member). Adiponectin contains a modular structure comprising an N-terminal collagenous domain followed by a C-terminal globular domain (gAcrp30). Adiponectin plays a role in various physiological processes such as energy homeostasis and obesity. Plasma levels of adiponectin are reduced in obese humans, and decreased levels are associated with insulin resistance and hyperinsulinemia.
• Synonyms: ADIPOQ, ACDC, ACRP30, APM1, GBP28