DYKDDDDK Epitope Tag Mouse Monoclonal Antibody [Clone ID: F-tag-01]

CAT#: SM3146P

DYKDDDDK Epitope Tag mouse monoclonal antibody, clone F-tag-01, Purified

Product Images

Confocal microscopy of COS-7 cells transfected with expression constructs encoding fusion nuclear protein with DDDDK epitope. A - fusion nuclear protein (red) stained with purified anti-DDDDK (F-tag-01) (detection by Goat anti-mouse IgG1 Alexa Fluor 594) B - cell nuclei stained with DAPI (blue) C - merged figures - confirmation of nuclear localization of the fusion protein; cell nuclei stained with DAPI (blue)

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  Confocal microscopy of COS-7 cells transfected with expression constructs encoding fusion nuclear protein with DDDDK epitope. A - fusion nuclear protein (red) stained with purified anti-DDDDK (F-tag-01) (detection by Goat anti-mouse IgG1 Alexa Fluor 594) B - cell nuclei stained with DAPI (blue) C - merged figures - confirmation of nuclear localization of the fusion protein; cell nuclei stained with DAPI (blue)

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  Confocal microscopy of COS-7 cells transfected with expression constructs encoding fusion nuclear protein with DDDDK epitope. A - fusion nuclear protein (red) stained with purified anti-DDDDK (F-tag-01) (detection by Goat anti-mouse IgG1 Alexa Fluor 594) B - cell nuclei stained with DAPI (blue) C - merged figures - confirmation of nuclear localization of the fusion protein; cell nuclei stained with DAPI (blue)

Specifications

Product Data

• Clone Name: F-tag-01
• Applications: IF, IP, WB
• Recommended Dilution: Western blot.
  Immunoprecipitation.
  Immunocytochemistry.
• Reactivities: All Species
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Synthetic peptide DYKDDDDK conjugated to KLH
• Specificity: The antibody F-tag-01 recognizes DDDDK-tagged proteins in all species.
• Formulation: Phosphate buffered saline (PBS), pH~7.4, with 15 mM Sodium Azide as preservative.
  State: Purified
  State: Liquid purified Ig fraction (> 95% by SDS-PAGE)
• Concentration: lot specific
• Purification: Affinity Chromatography on Protein A
• Conjugation: Unconjugated
• Storage: Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Synonyms: FLAG Epitope Tag, ECS Epitope Tag, FLAG-tag, ECS-tag, D-tag
• Note: The small size of this tag and its high hydrophilicity decrease the probability of interference with its expression, proteolytic maturation, antigenicity, localization and function.