CSK Mouse Monoclonal Antibody [Clone ID: CSK-04]

CAT#: SM3138P

CSK mouse monoclonal antibody, clone CSK-04, Purified

Product Images

Immunofluorescence staining of Csk in human HeLa cell line using anti-Csk (CSK-04; green). Actin cytoskeleton was decorated by phalloidin (red) and cell nuclei stained with DAPI (blue).

Specifications

Product Data

• Clone Name: CSK-04
• Applications: IF, IP, WB
• Recommended Dilution: Immunoprecipitation.
  Western Blotting (1:250-1:300. Weak non-specific band of cca 60 kDa is detected in human cell lines in lower antibody dilutions).
  Immunochemistry (Positive control: HeLa human cervix carcinoma cell line).
• Reactivities: Human, Mouse
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Bacterially expressed recombinant fragment of human Csk corresponding to aa 330-450.
• Specificity: The antibody CSK-04 reacts with Csk (C-terminal Src kinase), a 50 kDa non-receptor protein tyrosine kinase expressed in cytosol.
• Formulation: Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.4
  State: Purified
  State: Liquid Ig fraction
• Purification: Protein A affinity chromatography; purity: > 95% (by SDS-PAGE)
• Conjugation: Unconjugated
• Storage: Store the antibody at 2 - 8 °C up to one month or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Gene Name: c-src tyrosine kinase
• Database Link:
  Entrez Gene 1445 Human
  UniProt ID P41240
• Background: C-terminal Src kinase (Csk) is a non-receptor protein tyrosine kinase which resembles Src-family kinases, but unlike them lacks the conserved autophosphorylation site, the regulatory C-terminal tyrosine as well as myristylation and palmitylation. Csk negatively regulates Src-family kinases by phosphorylation of their C-terminal regulatory tyrosine.
  Disruption of the csk gene causes constitutive activation of Src-family kinases, and overexpression of Csk usually counteracts their signaling. The Csk-mediated regulation of
  those Src-family kinases that are localized in lipid rafts is enabled by a ubiquitously expressed transmembrane adaptor PAG (also known as Cbp, Csk-binding protein), which recruits Csk.
• Synonyms: Tyrosine-protein kinase CSK, C-SRC kinase, Protein-tyrosine kinase CYL