CD71 (TFRC) Mouse Monoclonal Antibody [Clone ID: MEM-75]

CAT#: SM3104B

CD71 (TFRC) mouse monoclonal antibody, clone MEM-75, Biotin

Product Images

Immunofluorescence staining of CD71 in Human HeLa cell line using anti-CD71 antibody (MEM-75; green). Actin cytoskeleton decorated by phalloidin (red) and cell nuclei stained with DAPI (blue).

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  Surface staining of NALM-6 human peripheral blood pre-B cell leukemia cell line with anti-CD71 (MEM-75) biotin; detection by Streptavidin-PE. Total viable cells were used for analysis.

Specifications

Product Data

• Clone Name: MEM-75
• Applications: FC, IF
• Recommended Dilution: Indirect Immunofluorescence analysis by Flow Cytometry: 1/200.
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: NALM-6 Human pre-B cell line
• Specificity: The antibody MEM-75 reacts with CD71 antigen (Transferrin Receptor), a 95 kDa type II homodimeric transmembrane glycoprotein expressed on activated B and lymphocytes, macrophages and erythroid precursors; it is lost on resting blood leukocytes.
  The antibody MEM-75 does not block binding of Transferrin to the receptor.
• Formulation: PBS, pH~7.4 containing 15 mM Sodium Azide as preservative
  Label: Biotin
  State: Liquid purified IgG fraction
  Label: Conjugated with Biotin-LC-NHS under optimum conditions. The reagent is free of unconjugated biotin.
• Concentration: lot specific
• Conjugation: Biotin
• Storage: Store the antibody undiluted at 2 - 8 °C. DO NOT FREEZE!
• Stability: Shelf life: one year from despatch.
• Gene Name: transferrin receptor
• Database Link:
  Entrez Gene 7037 Human
  UniProt ID P02786
• Background: CD71 (transferrin receptor) is a type II transmembrane glycoprotein expressed as homodimer in erythroid blood cell line and in activated leukocytes. Upon binding of holotransferrin (complex of transferrin and iron ions), CD71 is internalized by clathrin-mediated endocytosis. Acidification of endosomes by vesicular membrane proton pumps leads to dissociation of iron ions, whereas transferrin (apotransferrin) remains associated with CD71 and recycles to the cell surface, where it is released upon exposure to normal pH. CD71 is also involved in uptake of non-transferrin bound iron.
• Synonyms: TfR1, p90, Transferrin receptor protein 1