CD18 (ITGB2) Mouse Monoclonal Antibody [Clone ID: MEM-48]

CAT#: SM3103P

CD18 (ITGB2) mouse monoclonal antibody, clone MEM-48, Purified

Product Images

Western Blotting analysis (non-reducing conditions) of whole cell lysate using anti-human CD18 (MEM-48). Lane 1: KG-1a human acute myeloid leukemia cell line;Lane 2: HPB-ALL human peripheral blood T cell leukemia cell line

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  Immunohistochemistry staining of human spleen (paraffin sections) using anti-CD18 (MEM-48).

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  Figure 1A. Surface staining of human peripheral blood cells with anti-human CD18 (MEM-48) PE. Cells in the granulocyte gate were used for analysis.

Specifications

Product Data

• Clone Name: MEM-48
• Applications: FC, FN, IHC, IP, WB
• Recommended Dilution: Flow cytometry: 2 µg/ml.
  Immunoprecipitation.
  Western blot (non-reducing conditions): 2 µg/ml. Positive Control: Kg-1a human leukemia cell lysate. Sample preparation: buffer with laurylmaltoside, 2 x non-reducing SDS. SDS-PAGE (6% separating gel, 4% stacking gel).
  Immunohistochemistry on paraffin sections: 10 µg/ml. Positive tissue: spleen, microglia.
  Immunohistochemistry on frozen sections.
  Functional Assays: The antibody induces high-affinity conformation of LFA-1 complex.
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Leucocytes of a patient suffering from a LGL-type leukaemia
• Specificity: The antibody recognizes an epitope involving residues 534-546 in cysteine-rich repeat 3 of the CD18 antigen (Integrin beta2 chain).
  CD18 is a 90-95 kDa type I transmembrane protein expressed on all leukocytes.
• Formulation: PBS, pH~7.4 with 15 mM Sodium Azide as preservative.
  State: Purified
  State: Liquid purified Ig fraction (> 95% pure by SDS-PAGE)
• Concentration: lot specific
• Purification: Purified by sequential steps of physicochemical fractionation (differential precipitation and solid-phase chromatography methods).
• Conjugation: Unconjugated
• Storage: Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Gene Name: integrin subunit beta 2
• Database Link:
  Entrez Gene 3689 Human
  UniProt ID P05107
• Background: CD18, integrin b2 subunit, forms heterodimers with four types of CD11 molecule to constitute leukocyte (b2) integrins: aLb2 (CD11a/CD18, LFA-1), aMb2 (CD11b/CD18,
  Mac-1, CR3), aXb2 (CD11c/CD18) and aDb2 (CD11d/CD18). In most cases, the response mediated by the integrin is a composite of the functions of its individual subunits. These
  integrins are essential for proper leukocyte migration, mediating intercellular contacts.
  Absence of CD18 leads to leukocyte adhesion deficiency-1; severe reduction of CD18 expression leads to the development of a psoriasiform skin disease. CD18 is also a target of Mannheimia (Pasteurella) haemolytica leukotoxin and is sufficient to mediate leukotoxin-mediated cytolysis.
• Synonyms: Integrin beta-2, MFI7