CD8A Mouse Monoclonal Antibody [Clone ID: MEM-31]

CAT#: SM3102P

CD8A mouse monoclonal antibody, clone MEM-31, Purified

Product Images

Surface staining (mass cytometry) of PBMC after Ficoll-Paque separation with anti-human CD8 (MEM-31) Sm152. Gated on singlets.

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  Surface staining of human peripheral blood lymphocytes using anti-human CD8 (clone MEM-31) PE

Specifications

Product Data

• Clone Name: MEM-31
• Applications: FC, IP
• Recommended Dilution: Flow Cytometry (1 µl/ml).
  Immunoprecipitation.
  The antibody does not react with formaldehyde-fixed cells; negative in Western Blotting application.
• Reactivities: Human
• Host: Mouse
• Isotype: IgG2a
• Clonality: Monoclonal
• Immunogen: Crude thymus membrane fraction
• Specificity: The antibody recognizes a conformationally-dependent epitope of CD8 antigen.
• Formulation: Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.4
  State: Purified
  State: Liquid Ig fraction
• Concentration: lot specific
• Purification: Protein-A affinity chromatography; purity: > 95 % (by SDS-PAGE)
• Conjugation: Unconjugated
• Storage: Store undiluted at 2-8°C.
  DO NOT FREEZE!
  
• Stability: Shelf life: one year from despatch.
• Gene Name: CD8a molecule
• Database Link:
  Entrez Gene 925 Human
  UniProt ID P01732
• Background: The CD8 T cell coreceptor (monomer approx. 32-34 kDa) is expressed as ab heterodimer on majority of MHC I-restricted conventional T cells and thymocytes and as aa homodimer on subsets of memory T cells, intraepithelial lymphocytes, NK cells and dendritic cells. Regulation of CD8b level on T cell surface seems to be an important mechanism to control their effector function. Assembly of CD8 a-b but not a-a dimers is connected with formation or localization to the lipid rafts. Recruiting triggered TCR complexes to these membrane microdomains as well as affinity of TCR to MHC I is modulated by CD8, thereby affecting the functional diversity of the TCR signaling.
• Synonyms: CD8 alpha chain, CD8A, MAL

Reference Data

• Protein Families: Adult stem cells, Druggable Genome, ES Cell Differentiation/IPS, Secreted Protein, Transmembrane
• Protein Pathways: Antigen processing and presentation, Cell adhesion molecules (CAMs), Hematopoietic cell lineage, Primary immunodeficiency, T cell receptor signaling pathway