CD42b / GPIbA Mouse Monoclonal Antibody [Clone ID: HIP1]

CAT#: SM3082P

CD42b / GPIbA mouse monoclonal antibody, clone HIP1, Purified

Product Images

Surface staining of human peripheral blood with anti-CD42b (HIP1) purified / GAM-APC.

Specifications

Product Data

• Clone Name: HIP1
• Applications: FC, FN, IHC
• Recommended Dilution: Flow Cytometry.
  Immunohistochemistry on Frozen Sections.
  Functional Application: The antibody inhibits the ristocetin-dependent binding of von Willebrand Factor (vWF) to platelets and ristocetin-induced platelet agglutination.
• Reactivities: Human, Primate
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Peripheral blood mononuclear cells of a patient suffering with CLL
• Specificity: CD42b antibody reacts with CD42b (GPIba), a 135-145 kDa membrane glycoprotein expressed on platelets and megakaryocytes. CD42b and CD42c (GPIbb) are composed in a disulfide linked heterodimer (CD42b/c; 160 kDa); CD42b/c forms a noncovalent complex with CD42a and CD42d.
• Formulation: Phosphate buffered saline (PBS) with 15 mM Sodium Azide as preservative, approx. pH 7.4
  State: Purified
  State: Liquid purified Ig fraction (> 95% by SDS-PAGE).
• Concentration: lot specific
• Purification: Protein A Chromatography
• Conjugation: Unconjugated
• Storage: Store undiluted at 2-8°C.
  DO NOT FREEZE!
  
• Stability: Shelf life: one year from despatch.
• Gene Name: glycoprotein Ib platelet alpha subunit
• Database Link:
  Entrez Gene 2811 Human
  UniProt ID P07359
• Background: CD42b (GPIba) composes together with GPIbb, GPIX and GPV the GPIb-IX-V receptor complex critical in the process of platelet-rich thrombus formation by tethering the platelet to a thrombogenic surface. CD42b binds to von Willebrand factor (VWF) exposed at a site of vascular injury, as well as to thrombin, coagulation factors XI and XII, high molecular wight kininogen, TSP-1, integrin Mac-1 and P-selectin. The extracellular domain of CD42b by its interactions also contributes to metastasis.
• Synonyms: Platelet glycoprotein Ib alpha chain, Glycoprotein Ibalpha, GP-Ib alpha, GPIb-alpha