CD105 (ENG) Mouse Monoclonal Antibody [Clone ID: MEM-226]

CAT#: SM3078R

CD105 (ENG) mouse monoclonal antibody, clone MEM-226, PE

Product Images

Surface staining of HUVEC (human umbilical vein endothelial cells) with anti-CD105 (MEM-226) PE. HEK-293 cells used as a negative control.

Specifications

Product Data

• Clone Name: MEM-226
• Applications: FC
• Recommended Dilution: Flow Cytometry analysis of Human blood cells using 20 µl reagent / 100 µl of whole blood or 10⁶ cells in a suspension.
  The content of a vial (2 ml) is sufficient for 100 tests.
• Reactivities: Human, Rat
• Host: Mouse
• Isotype: IgG2a
• Clonality: Monoclonal
• Immunogen: Recombinant Vaccinia virus containing the Human CD105 cDNA
• Specificity: This antibody reacts with CD105 (Endoglin), a 180 kDa type I homodimerizing membrane glycoprotein expressed on vascular endothelial cells (small and large vessels), activated monocytes and tissue macrophages, stromal cells of certain tissues including bone marrow, pre-B lymphocytes in fetal marrow and erythroid precursors in fetal and adult bone marrow; it is also present on syncytiotrophoblast on placenta throughout pregnancy.
• Formulation: PBS
  Label: PE
  State: Liquid purified Ig fraction
  Stabilizer: 0.2% (w/v) high-grade protease free BSA
  Preservative: 15 mM Sodium Azide
  Label: Conjugated with R-Phycoerythrin (PE) under optimum conditions. The conjugate is purified by size-exclusion chromatography and adjusted for direct use.
• Purification: Size-Exclusion Chromatography
• Conjugation: PE
• Storage: Store the antibody at 2-8°C.
  DO NOT FREEZE!
  This product is photosensitive and should be protected from light.
• Stability: Shelf life: one year from despatch.
• Gene Name: endoglin
• Database Link:
  Entrez Gene 2022 Human
  UniProt ID P17813
• Background: CD105 (Endoglin) is a homodimeric transmembrane glycoprotein serving in presence of TGFbR-2 as a receptor for TGFb-1 and TGFb-3. CD105 is highly expressed on endothelial cells and promotes angiogenesis during wound healing, infarcts and in a wide range of tumours and its gene expression is stimulated by hypoxia. CD105 prevents apoptosis in hypoxic endothelial cells and also antagonises the inhibitory effects of TGFb-1 on vascular endothelial cell growth and migration. Normal cellular levels of CD105 are required for formation of new blood vessels.
• Synonyms: ENG, END, HHT1, ORW, ORW1