CD8A Mouse Monoclonal Antibody [Clone ID: MEM-87]

CAT#: SM3038P

CD8A mouse monoclonal antibody, clone MEM-87, Purified

Product Images

Surface staining of CD8 in human peripheral blood with anti-CD8 (MEM-87) purified, GAM-APC.

Specifications

Product Data

• Clone Name: MEM-87
• Applications: FC, IP
• Recommended Dilution: Immunoprecipitation (Excellent for immunoisolation of CD8 + T cells).
  Flow Cytometry: 2 μg/ml.
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Leucocytes of a patient suffering from LGL Type Leukaemia
• Specificity: The antibody recognizes CD8, a cell surface glycoprotein found on most cytotoxic T lymphocytes that mediates efficient cell-cell interactions within the immune system.
• Formulation: Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.4
  State: Purified
  State: Liquid Ig fraction
• Concentration: lot specific
• Purification: Protein-A affinity chromatography; purity: > 95% (by SDS-PAGE)
• Conjugation: Unconjugated
• Storage: Store the antibody at 2 - 8 °C up to one month or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Gene Name: CD8a molecule
• Database Link:
  Entrez Gene 925 Human
  UniProt ID P01732
• Background: The CD8 T cell coreceptor (monomer approx. 32-34 kDa) is expressed as ab heterodimer on majority of MHC I-restricted conventional T cells and thymocytes and as aa homodimer on subsets of memory T cells, intraepithelial lymphocytes, NK cells and dendritic cells. Regulation of CD8b level on T cell surface seems to be an important mechanism to control their effector function. Assembly of CD8 a-b but not a-a dimers is connected with formation or localization to the lipid rafts. Recruiting triggered TCR complexes to these membrane microdomains as well as affinity of TCR to MHC I is modulated by CD8, thereby affecting the functional diversity of the TCR signaling.
  CD8 is a disulfide-linked dimer and exists as a CD8a homodimer or CD8a/b heterodimer (each monomer approx. 32-34 kDa).
• Synonyms: CD8 alpha chain, CD8A, MAL

Reference Data

• Protein Families: Adult stem cells, Druggable Genome, ES Cell Differentiation/IPS, Secreted Protein, Transmembrane
• Protein Pathways: Antigen processing and presentation, Cell adhesion molecules (CAMs), Hematopoietic cell lineage, Primary immunodeficiency, T cell receptor signaling pathway