CD5 Mouse Monoclonal Antibody [Clone ID: MEM-32]

CAT#: SM3027P

CD5 mouse monoclonal antibody, clone MEM-32, Purified

Specifications

Product Data

• Clone Name: MEM-32
• Applications: ELISA, FC, IF, IHC, IP, WB
• Recommended Dilution: Immunoprecipitation.
  Western blot (non reducing conditions): Laurylmaltoside lysing buffer, 1-2 μg/ml.
  Immunocytochemistry.
  Flow cytometry: 2 μg/ml.
  Immunohistochemistry (paraffin sections): 20 μg/ml.
  Positive tissue: spleen.
  ELISA: Can be used in the Sandwich ELISA as the capture antibody in pair with the detection antibody clone CRIS1.
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Crude thymus membrane fraction
• Specificity: The antibody MEM-32 reacts with the cell surface glycoprotein CD5, a 67kDa single-chain transmembrane glycoprotein expressed on mature T-lymphocytes, most of thymocytes and B-lymphocytes subset (B-1a lymphocytes).
• Formulation: Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.4
  State: Purified
  State: Liquid Ig fraction
• Concentration: lot specific
• Purification: Protein A affinity chromatography; purity: > 95% (by SDS-PAGE)
• Conjugation: Unconjugated
• Storage: Store the antibody at 2 - 8 °C up to one month or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Gene Name: CD5 molecule
• Database Link:
  Entrez Gene 921 Human
  UniProt ID P06127
• Background: CD5 antigen (T1; 67 kDa) is a human cell surface T-lymphocyte single-chain transmembrane glycoprotein. CD5 is expressed on all mature T-lymphocytes, most of thymocytes, subset of B-lymphocytes and on many T-cell leukemias and lymphomas. It is a type I membrane glycoprotein whose extracellular region contains three scavenger receptor cysteine-rich (SRCR) domains.
  The CD5 is a signal transducing molecule whose cytoplasmic tail is devoid of any intrinsic catalytic activity. CD5 modulates signaling through the antigen-specific receptor complex (TCR and BCR). CD5 crosslinking induces extracellular Ca++ mobilization, tyrosine phosphorylation of intracellular proteins and DAG production. Preliminary evidence shows protein associations with ZAP-70, p56lck, p59fyn, PC-PLC, etc. CD5 may serve as a dual receptor, giving either stimulatory or inhibitory signals depending both on the cell type and development stage. In thymocytes and B1a cells seems to provide inhibitory signals, in peripheral mature T lymhocytes it acts as a costimulatory signal receptor. CD5 is the phenotypic marker of a B cell subpopulation involved in the production of autoreactive antibodies.
  Disease relevance: CD5 is a phenotypic marker for some B cell lymphoproliferative
  disorders (B-CLL, Hairy cell leukemia, etc.). The CD5+ popuation is expanded in some
  autoimmune disorders (Rheumatoid Arthritis, etc.). Herpes virus infections induce loss of
  CD5 expression in the expanded CD8+ human T cells.
• Synonyms: CD5, LEU1